This paper presents an in-depth case study of Spore.fun, a real-world artificial life (ALife) evolution experiment leveraging Decentralized Physical Infrastructure Network (DePIN) technology. We highlight the failure of existing closed-system-based ALife simulations to achieve Out-of-Environmental Innovation (OEE) and analyze Spore.fun's approach, which builds an open-environment system through large-scale language model-based AI agents integrated with blockchain and a Trusted Execution Environment (TEE). In Spore.fun, agents manage their own social media accounts and cryptocurrency wallets, and interact directly with blockchain-based financial networks and human society. By analyzing the agents' behavior and evolutionary paths from a digital ethological perspective, we invite discussion on whether an open-environment ALife system motivated by economic incentives and based on permissionless computing can achieve OEE.