This paper addresses the challenges of quality assurance for large-scale language model (LLM) applications. We decompose LLM applications into three layers: the system shell layer, the prompt orchestration layer, and the LLM inference core layer, and evaluate the applicability of existing software testing methods to each layer. By analyzing the differences between testing methodologies in software engineering and AI, we identify six key challenges and propose four collaborative strategies (maintenance, transformation, integration, and runtime) to address them. Furthermore, we propose a closed-loop, reliable quality assurance framework that combines pre-deployment verification and runtime monitoring, as well as practical guidelines and protocols (AICL: Agent Interaction Communication Language) to support standardization and tooling for LLM application testing.