This paper proposes scSiameseClu, a novel Siamese Clustering framework for interpreting single-cell RNA sequencing (scRNA-seq) data. scSiameseClu was developed to address the challenges of scRNA-seq data analysis, such as noise, sparsity, and high dimensionality. scSiameseClu comprises three main steps: (1) Dual Augmentation Module (enhancing the robustness of representation learning through biologically informative perturbations), (2) Siamese Fusion Module (mitigating over-smoothing through cross-correlation improvement and adaptive information fusion), and (3) Optimal Transport Clustering (efficiently aligning cluster assignments to a predefined ratio using Sinkhorn distance). Evaluation results on seven real-world datasets show that scSiameseClu outperforms existing state-of-the-art methods.