This paper presents a detailed case study of Spore.fun, a real-world AI evolution experiment for achieving open evolution (OEE), based on a novel technical innovation of deploying AI agents based on large language models (LLMs) on a blockchain that integrates a decentralized physical infrastructure network (DePIN) and a trusted execution environment (TEE). Overcoming the limitations of conventional closed system simulations, we analyze the behaviors and evolutionary paths of agents pursuing autonomous and continuous innovation through interactions with blockchain-based financial networks and broader human social media from a digital zoological perspective. This prompts a discussion on whether an “open” ALife system driven by permissionless computing and economic incentives can achieve the long-term goal of OEE.