BD-tau, a BODIPY-based fluorescence sensor detects pathological tau aggregates in live cells. As a fluorescence turn-on sensor, the quantum yield of BD-tau increased 3.8-fold upon binding to tau aggregates in vitro. β-sheet protein panel assay indicates that BD-tau also responds to other β-sheet protein aggregates such as amyloid plaques and insulin aggregates. However, compared with other conventional tau probes, BD-tau has superior selectivity to tau aggregates over amyloid plaques or insulin aggregates. As a cell-permeable imaging probe, BD-tau selectively labeled pathological tau aggregates in live hippocampal neurons.
■ Cell Image
BD-tau labels tau aggregates in brain slices of a tau transgenic mouse model. The representative images of brain tissue stained with BD-tau and the anti-tau PHF antibody. Nuclei were counterstained with Hoechst. Scale bar = 100 mm.
■ Related probes: BD-Oligo, 2C40, 2E10
■ Known Property Tau probe ■ Application Immunofluorescence ■ Cell selectivity mechanism unknown
■ Reference
1.
Development of a BODIPY-based fluorescent probe for imaging pathological tau aggregates in live cells, Lim, S.; Haque, M. M.; Su, D.; Kim, D.; Lee, J. S.; Chang, Y. T.; Kim, Y. K. Chem. Commun. 2017, 53, 1607-1610.