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Activated macrophage probe

CDg16 [Catalog # P016]

■ Chemical structure & Information
CDg16 (Compound of Designation green 16) is a selective probe for activated macrophage over resting state cells, and is expected to have high signal with low background at active inflammation site. CDg16 was originally discovered from mouse macrophage cell line (Raw264.7), but also showed good selectivity in mouse primary macrophages and human cell line. The staining of CDg16 is in vesicle in the cell and partially overlapped with lysosome.
■ Cell Image
CDg16-stained activated macrophages (a) Mφ (non-activated macrophages) and lipopolysaccharide (LPS) and interferon-gamma (IFNɣ) activated M1 (classically activated macrophages) were used to examine the selectivity of CDg16. CDg16 stained only LPS and IFNɣ treated Raw264.7 cells. LPS and IFNɣ activated peritoneal macrophages (primary macrophages) were also stained by CDg16. (b) CDg16 signals colocalized with LysoTracker, which stains lysosomes
■ Related probes: CDnir7
■ Known Property
activated macrophage probe
■ Application
Immunofluorescence
■ Cell selectivity mechanism
GOLD (SLC18B1)
■ Reference
1.
Imaging inflammation using an activated macrophage probe with Slc18b1 as the activationselective gating target, Park, S. J.; Kim, B.; Choi, S.; Balasubramaniam, S.; Lee, S. C.; Lee, J. Y.; Kim, H. S.; Kim, J. Y.; Kim, J. J.; Lee, Y. A.; Kang, N. Y.; Kim, J. S.; Chang, Y. T. Nat. Commun. 2019, 10, 1111.

■ ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equvalent to 1 mM / 100 µL)

■ Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

■ General Use Guide
•
More than 1/100 dilution of 10mM of DMSO stock solution is essential
•
For biomedical use to avoid DMSO concentration higher than 1%.
•
Working concentrations for specific applications should be determined by the investigator.
•
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
■ Data sheet PDF file
CDg16 data sheet v0.2.pdf677.02KB
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