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Polyphosphate probes

JC-D7 [Catalog # P035]
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🔬PHOENIX FLUOR

JC-D8 [Catalog # P036]

■ Chemical structure & Information
JC-D7/ JC-D8 demonstrate high selectivity for the labeling of polyP that was not sensitive to a number of ubiquitous organic polyphosphates, notably RNA. Use of these probes allowed us to demonstrate the real time detection of polyP release from lysosomes in live cells.
■ Cell Image
Mitochondrially targeted GFP-labeled exopolyphosphatase (mgPPX) overexpression leads to increase activity of the enzyme and therefore to a decreased levels of polyphosphate in GFP-positive mitochondria. (B, inset) colocalization profile taken along the arrow showing that GFP and JC-D8 signal do not significantly overlap.
Known Property
Poly phosphate probe
Application
Live imaging, Immunofluorescence
Cell selectivity mechanism
unknown
Reference
1.
In Situ Investigation of Mammalian Inorganic Polyphosphate Localization Using Novel Selective Fluorescent Probes JC-D7 and JC-D8, Angelova, P. R.; Agrawalla, B. K.; Elustondo, P. A.; Gordon, J.; Shiba, T.; Abramov, A. Y.; Chang, Y. T.; Pavlov, E. V. ACS Chem Biol. 2014, 9, 2101-2110.

ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equvalent to 1 mM / 100 µL)

Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

General Use Guide
More than 1/100 dilution of 10mM of DMSO stock solution is essential
For biomedical use to avoid DMSO concentration higher than 1%.
Working concentrations for specific applications should be determined by the investigator.
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
Data sheet PDF file
JC-D8 data sheet v0.2.pdf668KB
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