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JC-D7 [Catalog # P035]
■ Chemical structure & Information
JC-D7/ JC-D8 demonstrate high selectivity for the labeling of polyP that was not sensitive to a number of ubiquitous organic polyphosphates, notably RNA. Use of these probes allowed us to demonstrate the real time detection of polyP release from lysosomes in live cells.
■ Cell Image
Overexpression of mitochondrially targeted, GFP-labeled polyphosphate kinase (mgPPK) results in increased level of JC-D7 fluorescence. (A, inset) Colocalization profile estimated from fluorescence intensity measured along the arrow, shows that areas with increased levels of mtPPK expression correspond to areas with increased levels of endogenous polyphosphate.
■ Known Property
Poly phosphate probe
■ Application
Live imaging, Immunofluorescence
■ Cell selectivity mechanism
unknown
Poly phosphate probe
■ Application
Live imaging, Immunofluorescence
■ Cell selectivity mechanism
unknown
■ Reference
1.
In Situ Investigation of Mammalian Inorganic Polyphosphate Localization Using Novel Selective Fluorescent Probes JC-D7 and JC-D8, Angelova, P. R.; Agrawalla, B. K.; Elustondo, P. A.; Gordon, J.; Shiba, T.; Abramov, A. Y.; Chang, Y. T.; Pavlov, E. V. ACS Chem Biol. 2014, 9, 2101-2110.
■ ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equivalent to 1 mM / 100 µL)
■ Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C
■ General Use Guide
•
More than 1/100 dilution of 10mM of DMSO stock solution is essential
•
For biomedical use to avoid DMSO concentration higher than 1%.
•
Working concentrations for specific applications should be determined by the investigator.
•
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
precipitated out from buffer solution.
■ Data sheet PDF file
JC-D7 data sheet.pdf505.30KB