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TP (Two Photon)-alpha [Catalog # P026]
■ Chemical structure & Information
TP-alpha (Two Photon alpha cell probe) was discovered from “two photon green fluorescence library” by cell-based screening. TP-alpha is a pancreatic alpha cell selective probe over beta cell or acinar cell. TP-alpha showed dose dependent fluorescence increase upon treatment of glucagon, with low response to insulin or other proteins.
■ Cell Image
Antibody confirmation of TP-α selectivity by imaging and flow-cytometry. (A) Cultured dissociated islets stained with 1 μM TPα (in green) followed by immunostaining with glucagon antibody (in red). Merged image demonstrates the TP-α stained cells to be glucagon positive alpha cells (white arrow). Scale bar is 50 μm. (B) Dual parametric dot plot for TP-α and glucagon antibody. About 17 000 islet cell counted.
■ Related probes: Glucagon yellow (GY)
■ Known Property
pancreatic alpha cell
■ Application
Immunofluorescence and 3D imaging of live alpha cells using two photon microscopy
■ Cell selectivity mechanism
POLD (glucagon)
pancreatic alpha cell
■ Application
Immunofluorescence and 3D imaging of live alpha cells using two photon microscopy
■ Cell selectivity mechanism
POLD (glucagon)
■ Reference
1.
Glucagon-secreting alpha cell selective two-photon fluorescent probe TP-α: for live pancreatic islet imaging, Agrawalla, B. K.; Chandran, Y.; Phue, W. H.; Lee, S. C.; Jeong, Y. M.; Wan, S. Y.; Kang, N. Y.; Chang, Y. T. J. Am. Chem. Soc. 2015, 137, 5355-5362.
■ ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equivalent to 1 mM / 100 µL)
■ Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C
■ General Use Guide
•
More than 1/100 dilution of 10mM of DMSO stock solution is essential
•
For biomedical use to avoid DMSO concentration higher than 1%.
•
Working concentrations for specific applications should be determined by the investigator.
•
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
precipitated out from buffer solution.
■ Data sheet PDF file
TP alpha data sheet v0.2.pdf656.45KB