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Alpha-cell probes

GY (Glucagon Yellow) [Catalog # P024]
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🔬PHOENIX FLUOR

TP (Two Photon)-alpha [Catalog # P026]

■ Chemical structure & Information
TP-alpha (Two Photon alpha cell probe) was discovered from “two photon green fluorescence library” by cell-based screening. TP-alpha is a pancreatic alpha cell selective probe over beta cell or acinar cell. TP-alpha showed dose dependent fluorescence increase upon treatment of glucagon, with low response to insulin or other proteins.
■ Cell Image
Antibody confirmation of TP-α selectivity by imaging and flow-cytometry. (A) Cultured dissociated islets stained with 1 μM TPα (in green) followed by immunostaining with glucagon antibody (in red). Merged image demonstrates the TP-α stained cells to be glucagon positive alpha cells (white arrow). Scale bar is 50 μm. (B) Dual parametric dot plot for TP-α and glucagon antibody. About 17 000 islet cell counted.
Related probes: Glucagon yellow (GY)
Known Property
pancreatic alpha cell
Application
Immunofluorescence and 3D imaging of live alpha cells using two photon microscopy
Cell selectivity mechanism
POLD (glucagon)
Reference
1.
Glucagon-secreting alpha cell selective two-photon fluorescent probe TP-α: for live pancreatic islet imaging, Agrawalla, B. K.; Chandran, Y.; Phue, W. H.; Lee, S. C.; Jeong, Y. M.; Wan, S. Y.; Kang, N. Y.; Chang, Y. T. J. Am. Chem. Soc. 2015, 137, 5355-5362.

ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equvalent to 1 mM / 100 µL)

Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

General Use Guide
More than 1/100 dilution of 10mM of DMSO stock solution is essential
For biomedical use to avoid DMSO concentration higher than 1%.
Working concentrations for specific applications should be determined by the investigator.
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
Data sheet PDF file
TP alpha data sheet v0.2.pdf672KB
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