Home
SENPRO
ABOUT US
🔬PROBES
FAQ
Subscribe

B cell probe

CDgB [Catalog # P032]
Copyright© All Rights Reserved.
🔬PHOENIX FLUOR

CDgB [Catalog # P032]

■ Chemical structure & Information
CDgB (Compound of Designation green for B cell) is a selective fluorescent probe for B cell over T cell. B and T lymphocytes are similar in size and shape and it is practically impossible to distinguish them without the aid of antibody. CDgB is the first small molecule probe which distinguish B cell from T cell. CDgB has long hydrocarbon chain and the cell selectivity originates from the cell membrane lipid composition. In comparing to B cell, T cell has longer hydrocarbon in the plasma membrane lipid and also higher contents of cholesterol.
■ Cell Image
Development of the B-cell-selective probe CDgB. (a) Fluorescence images of double staining for CDgB (left) and anti-CD3e (middle). The images (right) are merged with CDgB (FITC) and anti-CD3e (Cy5). The splenocytes that CDgB stains are not overlapped with anti-CD3e for T cells. Scale bar: 10 μm. (b) Flow cytometry dot-plot image of double staining with anti-CD3e (right) for T cells and CDgB (FITC) on splenocytes.
Known Property
B lymphocyte probe
Application
Immunofluorescence
Cell selectivity mechanism
LOLD (lipid membrane fluidity)
Reference
1.
Lipid-Oriented Live-Cell Distinction of B and T Lymphocytes, Kwon, H. Y.; Kumar Das, R.; Jung, G. T.; Lee, H. G.; Lee, S. H.; Berry, S. N.; Tan, J. K. S.; Park, S.; Yang, J. S.; Park, S.; Baek, K.; Park, K. M.; Lee, J. W.; Choi, Y. K.; Kim, K. H.; Kim, S.; Kim, K. P.; Kang, N. Y.; Kim, K.; Chang, Y. T.* J. Am. Chem. Soc. 2021, 143, 5836-5844.

ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equvalent to 1 mM / 100 µL)

Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

General Use Guide
More than 1/100 dilution of 10mM of DMSO stock solution is essential
For biomedical use to avoid DMSO concentration higher than 1%.
Working concentrations for specific applications should be determined by the investigator.
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
Data sheet PDF file
CDgB data sheet v0.2.pdf378KB
Made with SlashPage