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CDgB [Catalog # P032]
■ Chemical structure & Information
CDgB (Compound of Designation green for B cell) is a selective fluorescent probe for B cell over T cell. B and T lymphocytes are similar in size and shape and it is practically impossible to distinguish them without the aid of antibody. CDgB is the first small molecule probe which distinguish B cell from T cell. CDgB has long hydrocarbon chain and the cell selectivity originates from the cell membrane lipid composition. In comparing to B cell, T cell has longer hydrocarbon in the plasma membrane lipid and also higher contents of cholesterol.
■ Cell Image
Development of the B-cell-selective probe CDgB. (a) Fluorescence images of double staining for CDgB (left) and anti-CD3e (middle). The images (right) are merged with CDgB (FITC) and anti-CD3e (Cy5). The splenocytes that CDgB stains are not overlapped with anti-CD3e for T cells. Scale bar: 10 μm. (b) Flow cytometry dot-plot image of double staining with anti-CD3e (right) for T cells and CDgB (FITC) on splenocytes.
■ Known Property
B lymphocyte probe
■ Application
Immunofluorescence
■ Cell selectivity mechanism
LOLD (lipid membrane fluidity)
B lymphocyte probe
■ Application
Immunofluorescence
■ Cell selectivity mechanism
LOLD (lipid membrane fluidity)
■ Reference
1.
Lipid-Oriented Live-Cell Distinction of B and T Lymphocytes, Kwon, H. Y.; Kumar Das, R.; Jung, G. T.; Lee, H. G.; Lee, S. H.; Berry, S. N.; Tan, J. K. S.; Park, S.; Yang, J. S.; Park, S.; Baek, K.; Park, K. M.; Lee, J. W.; Choi, Y. K.; Kim, K. H.; Kim, S.; Kim, K. P.; Kang, N. Y.; Kim, K.; Chang, Y. T.* J. Am. Chem. Soc. 2021, 143, 5836-5844.
■ ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equivalent to 1 mM / 100 µL)
■ Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C
■ General Use Guide
•
More than 1/100 dilution of 10mM of DMSO stock solution is essential
•
For biomedical use to avoid DMSO concentration higher than 1%.
•
Working concentrations for specific applications should be determined by the investigator.
•
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
precipitated out from buffer solution.
■ Data sheet PDF file
CDgB data sheet v0.2.pdf369.41KB