RESEARCH PROJECT

All
circRNAs
Paper Reading
Pilot Study
Experimental methods
miRNA expression level
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
https://www.youtube.com/watch?v=guUSs-PyaCU
HB miR Multi Assay Kit (System I로 진행)
Western blot
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
protein expression level
Protein extraction
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
Protein quantification
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
Bradford assay
PCR cloning (for circRNA expression)
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
BIOFACT
KPNA3 regulates histone locus body formation by modulating condensation and nuclear import of NPAT
  1. Paper Reading
  • J
    Jina Amie
https://rupress.org/jcb/article/224/1/e202401036/277130/KPNA3-regulates-histone-locus-body-formation-by?searchresult=1
circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis
  1. circRNAs
  2. Pilot Study
  3. Paper Reading
  • J
    Jina Amie
Not Started
Chen D, Zhang Y, Meng L, Lu L, Meng G. circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis. Open Med (Wars). 2022 Dec 16;17(1):2085-2097. doi: 10.1515/med-2022-0536. PMID: 36578555; PMCID: PMC9761921.
2월 저널클럽 발표 논문

Protein quantification

Category
  1. circRNAs
  2. Experimental methods
메모
Bradford assay
Created by
  • J
    Jina Amie
Created at
▪︎ BSA standard box, Bradford 시약(5X): 4℃ 냉장고
▪︎ kit: 오른쪽 위에서 두번째 서랍
▪︎ [ BSA(5) + Blank (1) + Sample (n) ] x double check
▪︎ Each well: Mix 150µl + BSA standard or sample 5µl
▪︎ 순서: 1. Mix 만들고 well에 담기 → 2. 기계 켜두기 → 3. 시약 섞기 → 4. 측정
▪︎ shaking on 6번, wave length 595 설정 확인 후 → sample 담긴 well 체크 → finish 버튼 클릭
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