RESEARCH PROJECT

All
circRNAs
Paper Reading
Pilot Study
Experimental methods
miRNA expression level
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
https://www.youtube.com/watch?v=guUSs-PyaCU
HB miR Multi Assay Kit (System I로 진행)
Western blot
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
protein expression level
Protein extraction
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
Protein quantification
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
Bradford assay
PCR cloning (for circRNA expression)
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
BIOFACT
KPNA3 regulates histone locus body formation by modulating condensation and nuclear import of NPAT
  1. Paper Reading
  • J
    Jina Amie
https://rupress.org/jcb/article/224/1/e202401036/277130/KPNA3-regulates-histone-locus-body-formation-by?searchresult=1
circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis
  1. circRNAs
  2. Pilot Study
  3. Paper Reading
  • J
    Jina Amie
Not Started
Chen D, Zhang Y, Meng L, Lu L, Meng G. circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis. Open Med (Wars). 2022 Dec 16;17(1):2085-2097. doi: 10.1515/med-2022-0536. PMID: 36578555; PMCID: PMC9761921.
2월 저널클럽 발표 논문

Protein extraction

Category
  1. circRNAs
  2. Experimental methods
Created by
  • J
    Jina Amie
Created at
▪︎ protase inhibitor (6개): -20℃ 냉장고 ⇒ 상온 해동
PMSF, cocktail, phosphatase inhibitor (4개)
▪︎ lysis buffer (RIPA): 4℃ 냉장고 (부족할 경우: 750호 RIPA buffer에서 40µl aliquot)
▪︎ Mix) protase inhibitor (6개 각 1µl/100µl 로) + lysis buffer 200µl (90∅ dish cell 꽉 기준) ⇒ n+1 분량
ex. sample cell이 3개라면,
⇒ 4개 분량 만들기. lysis buffer 800µl + protase inhibitor 각 8µ씩 Mix 만든 후, 잘 섞어주고 cell pellet이 담긴 용기에 넣어준다.
1) Mix + cell pellet ⇒ 4℃ ice에서 15-30min incubation (중간에 1-2번 tapping or voltexing)
2) 4℃ centrifuge PROG 1
3) supernatant만 떠서 keeping (-80℃)
Made with Slashpage