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circRNAs
Paper Reading
Pilot Study
Experimental methods
miRNA expression level
circRNAs
Experimental methods
J
Jina Amie
2025/02/11 4:52 PM
https://www.youtube.com/watch?v=guUSs-PyaCU
HB miR Multi Assay Kit (System I로 진행)
Western blot
circRNAs
Experimental methods
J
Jina Amie
2025/02/04 4:55 PM
protein expression level
Protein extraction
circRNAs
Experimental methods
J
Jina Amie
2025/02/04 1:30 PM
Protein quantification
circRNAs
Experimental methods
J
Jina Amie
2025/02/04 1:02 PM
Bradford assay
PCR cloning (for circRNA expression)
circRNAs
Experimental methods
J
Jina Amie
2025/01/24 11:59 AM
BIOFACT
KPNA3 regulates histone locus body formation by modulating condensation and nuclear import of NPAT
Paper Reading
J
Jina Amie
2025/01/10 10:44 AM
https://rupress.org/jcb/article/224/1/e202401036/277130/KPNA3-regulates-histone-locus-body-formation-by?searchresult=1
circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis
circRNAs
Pilot Study
Paper Reading
J
Jina Amie
2025/01/04 7:50 PM
Not Started
Chen D, Zhang Y, Meng L, Lu L, Meng G. circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis. Open Med (Wars). 2022 Dec 16;17(1):2085-2097. doi: 10.1515/med-2022-0536. PMID: 36578555; PMCID: PMC9761921.
2월 저널클럽 발표 논문
Protein extraction
Category
circRNAs
Experimental methods
Created by
J
Jina Amie
Created at
2025/02/04 1:30 PM
▪︎ protase inhibitor (6개): -20℃ 냉장고 ⇒ 상온 해동
PMSF, cocktail, phosphatase inhibitor (4개)
▪︎ lysis buffer (RIPA): 4℃ 냉장고 (부족할 경우: 750호 RIPA buffer에서 40µl aliquot)
▪︎ Mix) protase inhibitor (6개 각 1µl/100µl 로) + lysis buffer 200µl (90∅ dish cell 꽉 기준) ⇒ n+1 분량
ex. sample cell이 3개라면,
⇒ 4개 분량 만들기. lysis buffer 800µl + protase inhibitor 각 8µ씩 Mix 만든 후, 잘 섞어주고 cell pellet이 담긴 용기에 넣어준다.
1) Mix + cell pellet ⇒ 4℃ ice에서 15-30min incubation (중간에 1-2번 tapping or voltexing)
2) 4℃ centrifuge PROG 1
3) supernatant만 떠서 keeping (-80℃)
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