RESEARCH PROJECT

All
circRNAs
Paper Reading
Pilot Study
Experimental methods
miRNA expression level
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
https://www.youtube.com/watch?v=guUSs-PyaCU
HB miR Multi Assay Kit (System I로 진행)
Western blot
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
protein expression level
Protein extraction
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
Protein quantification
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
Bradford assay
PCR cloning (for circRNA expression)
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
BIOFACT
KPNA3 regulates histone locus body formation by modulating condensation and nuclear import of NPAT
  1. Paper Reading
  • J
    Jina Amie
https://rupress.org/jcb/article/224/1/e202401036/277130/KPNA3-regulates-histone-locus-body-formation-by?searchresult=1
circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis
  1. circRNAs
  2. Pilot Study
  3. Paper Reading
  • J
    Jina Amie
Not Started
Chen D, Zhang Y, Meng L, Lu L, Meng G. circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis. Open Med (Wars). 2022 Dec 16;17(1):2085-2097. doi: 10.1515/med-2022-0536. PMID: 36578555; PMCID: PMC9761921.
2월 저널클럽 발표 논문

PCR cloning (for circRNA expression)

Category
  1. circRNAs
  2. Experimental methods
메모
BIOFACT
Created by
  • J
    Jina Amie
Created at
[TA cloning & Circular cloning]
DAY 1: T-vector ligation → Transformation → E.coli plate spreading (O/N)
DAY 2: Picking & LB broth inoculation (O/N)
DAY 3: midi prep or mini prep → Restriction (3h) → Gel elution → Ligation (O/N)
⇒ prep 결과물 전기 영동 확인 및 sequencing 확인
▪︎ restriction enzyme: Sac I, Age II
▪︎ cloning vextor (circular): #1 (DAN stock) 67번
DAY 4: Transformation → E.coli plate spreading (O/N)
DAY 5: Picking & LB broth inoculation (O/N)
DAY 6: midi prep or mini prep → Transfection (+ 7 DAYS)
⇒ prep 결과물 전기 영동 확인 및 sequencing 확인
[TA cloning]
[VT201,VT202-020] All in One™ PCR Cloning Kit.pdf389.55KB
Made with Slashpage