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circRNAs
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Pilot Study
Experimental methods

PCR cloning (for circRNA expression)

Category
  1. circRNAs
  2. Experimental methods
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BIOFACT
Created by
  • J
    Jina Amie
Created at
[TA cloning & Circular cloning]
DAY 1: T-vector ligation → Transformation → E.coli plate spreading (O/N)
DAY 2: Picking & LB broth inoculation (O/N)
DAY 3: midi prep or mini prep → Restriction (3h) → Gel elution → Ligation (O/N)
⇒ prep 결과물 전기 영동 확인 및 sequencing 확인
▪︎ restriction enzyme: Sac I, Age II
▪︎ cloning vextor (circular): #1 (DAN stock) 67번
DAY 4: Transformation → E.coli plate spreading (O/N)
DAY 5: Picking & LB broth inoculation (O/N)
DAY 6: midi prep or mini prep → Transfection (+ 7 DAYS)
⇒ prep 결과물 전기 영동 확인 및 sequencing 확인
[TA cloning]
[VT201,VT202-020] All in One™ PCR Cloning Kit.pdf399KB
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