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circRNAs
Paper Reading
Pilot Study
Experimental methods
miRNA expression level
circRNAs
Experimental methods
J
Jina Amie
2025/02/11 4:52 PM
https://www.youtube.com/watch?v=guUSs-PyaCU
HB miR Multi Assay Kit (System I로 진행)
Western blot
circRNAs
Experimental methods
J
Jina Amie
2025/02/04 4:55 PM
protein expression level
Protein extraction
circRNAs
Experimental methods
J
Jina Amie
2025/02/04 1:30 PM
Protein quantification
circRNAs
Experimental methods
J
Jina Amie
2025/02/04 1:02 PM
Bradford assay
PCR cloning (for circRNA expression)
circRNAs
Experimental methods
J
Jina Amie
2025/01/24 11:59 AM
BIOFACT
KPNA3 regulates histone locus body formation by modulating condensation and nuclear import of NPAT
Paper Reading
J
Jina Amie
2025/01/10 10:44 AM
https://rupress.org/jcb/article/224/1/e202401036/277130/KPNA3-regulates-histone-locus-body-formation-by?searchresult=1
circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis
circRNAs
Pilot Study
Paper Reading
J
Jina Amie
2025/01/04 7:50 PM
Not Started
Chen D, Zhang Y, Meng L, Lu L, Meng G. circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis. Open Med (Wars). 2022 Dec 16;17(1):2085-2097. doi: 10.1515/med-2022-0536. PMID: 36578555; PMCID: PMC9761921.
2월 저널클럽 발표 논문
PCR cloning (for circRNA expression)
Category
circRNAs
Experimental methods
메모
BIOFACT
Created by
J
Jina Amie
Created at
2025/01/24 11:59 AM
[TA cloning & Circular cloning]
DAY 1: T-vector ligation → Transformation → E.coli plate spreading (O/N)
DAY 2: Picking & LB broth inoculation (O/N)
DAY 3: midi prep or mini prep → Restriction (3h) → Gel elution → Ligation (O/N)
⇒ prep 결과물 전기 영동 확인 및 sequencing 확인
▪︎ restriction enzyme: Sac I, Age II
▪︎ cloning vextor (circular): #1 (DAN stock) 67번
DAY 4: Transformation → E.coli plate spreading (O/N)
DAY 5: Picking & LB broth inoculation (O/N)
DAY 6: midi prep or mini prep → Transfection (+ 7 DAYS)
⇒ prep 결과물 전기 영동 확인 및 sequencing 확인
[TA cloning]
[VT201,VT202-020] All in One™ PCR Cloning Kit.pdf
399KB
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