# PiF [Catalog # P031]

**■ Chemical structure & Information**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/223320_GPwLa9lbz5JewXbUQn?q=80&s=1280x180&t=outside&f=webp)

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/223335_LC8j1IKYSqCsVqKHW7?q=80&s=1280x180&t=outside&f=webp)

**PiF **(Pancreatic islet Fluorinated probe) is a superior pancreatic beta cell probe over PiY, facilitating the pancreatic tissue preparation from day to hours. PiF could detect not only healthy pancreatic beta cells, but also transplanted islet in the liver. PiF has built in F atom which could be replaced with 18F for PET imaging. PET imaging of pancreatic islet was demonstrated by replacing 19F with 18F of PiF

**■ Cell Image**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/223358_m07GniM4KQ80QafP6i?q=80&s=1280x180&t=outside&f=webp)

(A) Fluorescent images were acquired after the cell culture of isolated islets. (Left) Merged fluorescent images of PiF (λex = 535 nm, λem = 585 nm, red) and TPα (λex = 370 nm, λem = 475 nm, green). (Middle) Merged confocal images of PiF (red) and anti-insulin (green: pseudocolor). Anti-insulin was imaged under a Cy5 filter. (Right) Merged confocal images of PiF (red) and anti-glucagon (green: pseudocolor). Anti-glucagon was imaged under a Cy5 filter. Hoechst was used for nuclei staining. (B) In vivo PET/CT images of [ 18F]PiF in ICR mice. ICR mice (n = 3) were intravenously dosed with approximately 7.4 MBq of [ 18F]PiF.

■ **Related probes**: PiY, TP-beta

■ **Known Property**
pancreatic beta cell probe
■ **Application**
Immunofluorescence and PET imaging
■ **Cell selectivity mechanism**
POLD (insulin)

■ **Reference**

1. **Multimodal imaging probe development for pancreatic β-cells: from fluorescence to PET**, Kang, N. Y.; Lee, J.; Lee, S. H.; Song, I. H.; Hwang, Y. H.; Kim, M. J.; Phue, W. H.; Agrawalla, B. K.; Wan, S. Y. D.; Lalic, J.; Park, S. J.; Kim, J. J.; Kwon, H. Y.; Im, S. H.; Bae, M. A.; Ahn, J. H.; Lim, C. S.; Teo, A. K. K.; Park, S.; Kim, S. E.; Lee, B. C.; Lee, D. Y._; Chang, Y. T._ J. Am. Chem. Soc. 2020, 142, 3430-3439.

■ **ORDER**

**International**

**USD 350 + VAT (10%) + Fedex delivery charge**

**In Korea**

**KRW 450,000 + VAT (10%)**

**100 nmol** (dried form: equivalent to 1 mM / 100 µL)

[order@senprobe.com](mailto:order@senprobe.com)

■ **Storage**
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

■ **General Use Guide**

- More than 1/100 dilution of 10mM of DMSO stock solution is essential

-  For biomedical use to avoid DMSO concentration higher than 1%.

- Working concentrations for specific applications should be determined by the investigator.

- It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
- precipitated out from buffer solution.

■ **Data sheet PDF file**

PiF data sheet v0.2.pdf

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