Sign In

RA-Resa NADH [Catalog #P046]

■ Chemical structure & Information
RA-Resa NADH is a turn-on fluorescent probe for direct NADH detection at physiological pH (7.4). It shows strong fluorescence enhancement with NADH, while exhibiting minimal response to common biomolecules (including NAD⁺) and a much weaker response to NADPH (λ_ex/λ_em = 480/570 nm).
■ Cell Image
Microscopic images of probe RA-Resa (5 μM, 15 min)-stained live OSCC cells after incubation in following conditions. (A,D) No glucose for 30 min; (B) 1 mM glucose for 30 min; (C) 20 mM glucose for 30 min; (E) 10 mM lactate for 10 min; (F) 10 mM lactate and 5 mM pyruvate for 10 min. Scale bar: 50 μm.
■ Known Property
Turn-on fluorescent NADH probe
■ Application
Fluorescence imaging of intracellular NADH
■ Selectivity mechanism
NADH-dependent turn-on
■ Reference
1.
Boronic Acid: A Bio-Inspired Strategy To Increase the Sensitivity and Selectivity of Fluorescent NADH Probe, *Wang, L.; Zhang, J.; Kim, B.; Peng, J.; Berry, S. N.; Ni, Y.; Su, D.; Lee, J.; Yuan, L.; Chang, Y.-T. ** J. Am. Chem. Soc. 2016, 138, 10394–10397.

■ ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equivalent to 1 mM / 100 µL)

■ Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

■ General Use Guide
•
More than 1/100 dilution of 10mM of DMSO stock solution is essential
•
For biomedical use to avoid DMSO concentration higher than 1%.
•
Working concentrations for specific applications should be determined by the investigator.
•
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
■ Data sheet PDF file
RA-Resa NADH Data sheet v1.0.pdf533.83KB
Made with Slashpage