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Tamed click reagent

CO-1 [Catalog # P049]
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🔬PHOENIX FLUOR

CO-1 [Catalog # P049]

■ Chemical structure & Information
CO-1 is cell permeable background-free probe through optimized lipophilicity, water solubility and charged van der Waals surface area. CO-1, a cyclooctyne-containing BODIPY probe, is also specifically label intracellular azide-tagged organelles & engineered proteins with minimum background.
■ Cell Image
Fluorescence imaging of mitochondria, lysosome and golgi apparatus in U-2 OS cells labelled with CO-1. Cells pre-treated with TPP-Az, Morph-Az or Sphingo-Az were incubated with 2 μM CO-1 in growth media at 37 °C for 1 h and followed by counterstaining with organelle trackers. Scale bar, 15 μm. (iv) Fluorescence imaging of histone H2B in live U-2 OS. U-2 OS cells were co-transfected with plasmids pIre-Azi3 and pmH2B-6-mKate2-16tag to incorporate UAA Azi into H2B-mKate2 at site 16. After Azi incorporation, cells were labelled with 10 μM CO-1 for 90 min at 37 °C. Cells were then washed and imaged. The mKate2 (red) signals were detected in cell nuclei only, and colocalized with the CO-1 (green) signals in the merged image. Scale bar, 5 μm.
Related probes: AzG-1
Known Property
Bio-orthogonal Click reaction
Application
Immunofluorescence
Cell selectivity mechanism
unknown
Reference
1.
Development of background-free tame fluorescent probes for intracellular live cell imaging, Alamudi, S. H.; Satapathy, R.; Kim, J.; Su, D.; Ren, H.; Das, R.; Hu, L.; Alvarado-Martínez, E.; Lee, J. Y.; Hoppmann, C.; Peña-Cabrera, E.; Ha, H. H.; Park, H. S.; Wang, L.; Chang, Y. T.* Nat. Commun. 2016, 7, 11964.

ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equvalent to 1 mM / 100 µL)

Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

General Use Guide
More than 1/100 dilution of 10mM of DMSO stock solution is essential
For biomedical use to avoid DMSO concentration higher than 1%.
Working concentrations for specific applications should be determined by the investigator.
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
Data sheet PDF file
CO-1 data sheet v0.2.pdf727KB
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