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ER Thermometers

ER ThermAC [Catalog # P037]

ER ThermAC [Catalog # P037]

■ Chemical structure & Information
A novel BODIPY-based thermosensitive dye, ER thermAC, is quickly and easily taken up into the endoplasmic reticulum of adipocytes where it forms a contiguous intraorganellar thermometer for the optical visualisation of thermogenesis. Following adrenergic stimuli, heat production occurred at random timings in each cell, and the dynamics of thermogenesis was consistent with mitochondrial depolarisation observed using JC-1.
■ Cell Image
ERthermAC targets the endoplasmic reticulum in adipocytes, and its intensity is inversely proportional to temperature. (A) ERthermAC (red) co-localises with ER-Tracker Green (green) in WT-1 cells, as evident from the yellow mix colour after superimposition of signals. Scale bar: 10 μm. (B) Chemical structure of ERthermAC. (C) Differentiated WT-1 cells were stained with ERthermAC, fixed with 4% formaldehyde, and imaged at different temperatures ranging between 18 °C and 43 °C. At higher temperatures, cells display lower ERthermAC fluorescence intensity. Scale bar: 20 μm. (D) Calibration curve shows a reversible non-linear relationship between temperature and fluorescence intensity of ERthermAC in fixed WT-1 cells between 18 °C and 43 °C. Temperature sensitivities determined by the linear fit were −1.07%/°C (R2 = 0.76, n = 15 cells) between 18.1 °C and 35.0 °C, and −4.76%/°C (R2 = 0.83, n = 15 cells) between 37 °C and 43 °C.
Related probes: ER Thermo Yellow, JC-1
Known Property
Temperature Probe
Application
Cell organelle imaging
Cell selectivity mechanism
unknown
Reference
1.
Optical visualisation of thermogenesis in stimulated single cell brown adipocytes, Kriszt, R.; Arai, S.; Itoh, H.; Lee, M. H.; Goralczyk, A. G.; Ang, X. M.; Cypess, A. M.; White, A. P.; Shamsi, F.; Xue, R.; Lee, J. Y.; Lee, S. C.; Hou, Y.; Kitaguchi, T.; Sudhaharan, T.; Ishiwata, S.; Lane, E. B.; Chang, Y. T. Tseng, Y. H.; Suzuki, M.; Raghunath, M. Sci. Rep. 2017, 7, 1383.ORDER

ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equvalent to 1 mM / 100 µL)

Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

General Use Guide
More than 1/100 dilution of 10mM of DMSO stock solution is essential
For biomedical use to avoid DMSO concentration higher than 1%.
Working concentrations for specific applications should be determined by the investigator.
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
Data sheet PDF file
ER ThermAC data sheet v0.2.pdf1.899894MB
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