# CDy9 [Catalog # P009]

■ **Chemical structure & Information**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/212900_GvmKoRJDK8fFCGYXu5?q=80&s=1280x180&t=outside&f=webp)

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/212916_ATxCIpjbubK9bxIRHW?q=80&s=1280x180&t=outside&f=webp)

**CDy9** (Compound of Designation yellow 9) is a highly selective mouse embryonic stem cell (mES) probe. The selectivity of **CDy9** was reduced when the chloroacetyl group was removed, while acetyl derivative maintained the selectivity.

■ **Cell Image**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/213015_XX98jPOlWW3Nqnbt1N?q=80&s=1280x180&t=outside&f=webp)

**CDy9** is a novel and versatile mESC-specific fluorescent probe. (a)Chemical structure of CDy9. (b) CDy9 selectively stains mESC over MEF. Both mESC and MEF were incubated with 1 mM CDy9 and imaged under the fluorescence microscope after 1 h. Scale bar: 25mm. (c) Flow cytometry analysis of mESC and MEF after incubation with **CDy9**. The fluorescence intensity of mESC upon treatment with **CDy9** is brighter than in MEF (right dot plot) and in unstained mESC (left dot plot).

■ **Related probes**: CDy1, CDr3, CDb8 

■ **Known Property**
mouse embryonic stem cell probe
■ **Application**
Immunofluorescence
■ **Cell selectivity mechanism**
GOLD (SLC13A5)

■ **Reference**

1. **A highly selective fluorescent probe for direct detection and isolation of mouse embryonic stem cells**, Chandran, Y.; Kang, N. Y.; Park, S. J.; Husen, A. S.; Kim, J. Y.; Sahu, S.; Su, D.; Lee, J.; Vendrell, M._; Chang, Y. T._ Bioorg. Med. Chem. Lett. 2015, 25, 4862-4865.

2. **A fluorescent chemical probe CDy9 selectively stains and enables the isolation of live naïve mouse embryonic stem cells**, Cho, S. J.; Kim, K. T.; Kim, J. S.; Kwon, O. S.; Go, Y. H.; Kang, N. Y.; Heo, H.; Kim, M. R.; Han, D. W.; Moon, S. H.; Chang, Y. T._; Cha, H. J._ Biomaterials 2018, 180, 12-23.

■ **ORDER**

**International**

**USD 350 + VAT (10%) + Fedex delivery charge**

**In Korea**

**KRW 450,000 + VAT (10%)**

**100 nmol** (dried form: equivalent to 1 mM / 100 µL)

[order@senprobe.com](mailto:order@senprobe.com)

■ **Storage**
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

■ **General Use Guide**

- More than 1/100 dilution of 10mM of DMSO stock solution is essential

-  For biomedical use to avoid DMSO concentration higher than 1%.

- Working concentrations for specific applications should be determined by the investigator.

- It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
- precipitated out from buffer solution.

■ **Data sheet PDF file**

CDy9 data sheet v0.2.pptx.pdf

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