# CDy5 [Catalog # P005]

■ **Chemical structure & Information**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/213338_kzfx1NTBXJT2n56gDh?q=80&s=1280x180&t=outside&f=webp)

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/213352_rH50i1iLLJnbFJIEpZ?q=80&s=1280x180&t=outside&f=webp)

**CDy5** selectively stains neural stem cells in neurosphere. In symmetric division, two identical daughter cells were visualized by **CDy5**. In asymmetric division, only small stem cell is fluorescently labeled and the other bigger differentiated cell was not stained by **CDy5**. The binding target of **CDy5** is acid ceramidase

■ **Cell Image**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/213441_NkU6465A30BWjD3YA7?q=80&s=1280x180&t=outside&f=webp)

(a) Confocal fluorescence micrograph (taken using A1R+ si, Nikon) of a **CDy5**-stained neuro sphere fixed in paraformaldehyde. Scale bar, 10 mm. (b) Chemical structure of **CDy5**. (c) Epifluorescence micrograph of living single cells dissociated fromCDy5-stainedneurospheres.**CDy5**-stainedcells are marked by white arrows. Scale bar, 10mm.(d) Neuro sphere assay of FACS sortedCDy5bright and CDy5 dim cells. Data represent mean  SD. * p < 0.01, Student’s t-test.

■ **Related probes**: CDy1, CDr3

■ **Known Property**
neural stem cells dye
■ **Application**
Immunofluorescence
■ **Cell selectivity mechanism**
POLD (acid ceramidase)

■ **Reference**

1. **A fluorescent probe for imaging symmetric and asymmetric cell division in neurosphere formation**, Yun, S. W.; Leong, C.; Bi, X.; Ha, H. H.; Yu, Y. H.; Tan, Y. L.; Narayanan, G.; Sankaran, S.; Kim, J. Y.; Hariharan, S.; Ahmed, S._; Chang, Y. T._ Chem. Commun. 2014, 50, 7492-7494.

■ **ORDER**

**International**

**USD 350 + VAT (10%) + Fedex delivery charge**

**In Korea**

**KRW 450,000 + VAT (10%)**

**100 nmol** (dried form: equivalent to 1 mM / 100 µL)

[order@senprobe.com](mailto:order@senprobe.com)

■ **Storage**
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

■ **General Use Guide**

- More than 1/100 dilution of 10mM of DMSO stock solution is essential

-  For biomedical use to avoid DMSO concentration higher than 1%.

- Working concentrations for specific applications should be determined by the investigator.

- It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
- precipitated out from buffer solution.

■** Data sheet PDF file**

CDy5 data sheet v0.2.pdf

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