# CDb8 [Catalog # P008]

■ **Chemical structure & Information**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/212503_hEMRQv6aEIjfpeTppN?q=80&s=1280x180&t=outside&f=webp)

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/212515_wiiDLkx4v5MaDYDn8u?q=80&s=1280x180&t=outside&f=webp)

**CDb8** (Compound of Designation blue 8) is a xanthone based blue fluorescent probe for mouse embryonic stem cell (mES). **CDb8** stains the outside of mES colony, i.e. glycocalyx area [1]. Glycocalyx is enriched with glycoproteins and glycolipids. The co-staining with related ES probes showed that CDy1 stains inside ES colony, and CDg4 and **CDb8** stains the surface of ES colony [2]. Based on the imaging data, the binding target of **CDb8** may be glycogen in glycocalyx.

■** Cell Image**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/212616_VoubNEsU7vf4KWKEec?q=80&s=1280x180&t=outside&f=webp)

Selective staining of mESC by **CDb8.** (a) Chemical structure of CDb8; (b) mESC was selectively stained by CDb8 at 1 mM for 1 h. Upper panel: mouse embryonic fibroblasts (MEF), middle: mouse embryonic stem cells (mESC), lower panel: mESC on MEF feeder. (c) Flow cytometry analysis of DMSO control cells. (d) Flow cytometry analysis of **CDb8** stained cells. The cells are loaded after 1 h incubation at 1 mM. B.F: bright field, scale bar: 100 mm.

■ R**elated probes: **CDy1, CDg4, CDy9

■ **Known Property**
mouse embryonic stem cell (mES) probe

■ **Application**
Immunofluorescenec

■ **Cell selectivity mechanism**
COLD (maybe glycogen)

■ **Reference**

1. **Solid phase combinatorial synthesis of xanthone library using click chemistry and its application to embryonic stem cell probe**, Ghosh, K. K; Ha, H. H.; Kang, N. Y.; Chandran, Y.; Chang, Y. T.* Chem. Commun. 2011, 47, 7488-7490

2. **Development of fluorescent Chalcone library and its application in the discovery of a mouse embryonic stem cell probe**, Lee, S. C.; Kang, N. Y.; Park, S. J.; Yun, S. W.; Chandran, Y.; Chang, Y. T.* Chem. Commun. 2012, 48, 6681-6683.

■ **ORDER**

**International:**

**USD 350 + VAT (10%) + Fedex delivery charge**

**In Korea:**

**KRW 450,000 + VAT (10%)**

**100 nmol** (dried form: equivalent to 1 mM / 100 µL)

[order@senprobe.com](mailto:order@senprobe.com)

■ **Storage**
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

■ **General Use Guide**

- More than 1/100 dilution of 10mM of DMSO stock solution is essential

-  For biomedical use to avoid DMSO concentration higher than 1%.

- Working concentrations for specific applications should be determined by the investigator.

- It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
- precipitated out from buffer solution.

■** Data sheet PDF file**

CDb8 data sheet v0.2.pdf

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