# CDb12 [Catalog # P012]

**■ Chemical structure & Information**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/221033_J2kQXEF9hZwJqC9hZT?q=80&s=1280x180&t=outside&f=webp)

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/221049_EUz1pmo6qJQzN1wSwA?q=80&s=1280x180&t=outside&f=webp)

**CDb12 **(Compound of Designation blue 12) was discovered from mitotic cell selective dye screening in RPE1 cells, and stains mitotic cells 7 times stronger than interphase cells. CDb12 stains nucleus and increase the fluorescence upon treatment of DNA, but not of RNA. **CDb12** could monitor the dynamic cell division at least for 2 cell division cycle. In contrast, Hoechst treated cells showed cell cycle arrest at M phase, may be due to too strong binding to DNA

**■ Cell Image**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/221126_hKBvgTf62csxaHgmr6?q=80&s=1280x180&t=outside&f=webp)

Time-lapse imaging of **CDb12**, compared to Hoechst 33342. RPE1 cells were stained with **CDb12 **(1 mM) or Hoechst 33342 (0.5 mg mL1 ). The mitosis was monitored for 24 h with frames taken every 3 min on a timelapse imaging system Nikon Biostation IM (Nikon) with DAPI filter (405 nm).

■ **Related probes: **CDy6

■ **Known Property**
mitotic cell probe
■ **Application**
Immunofluorescence
■ **Cell selectivity mechanism**
Unknown, neuclear localization 

■ **Reference**

1. **Development of nucleus staining fluorescent probe for dynamic mitosis imaging in live cells**, Ghosh, K. K.; Jeong, Y. M.; Kang, N. Y.; Lee, J. Y.; Wan, S. Y.; Kim, J. Y.; Yoo, J.; Kim, D.; Kim, Y. K._; Chang, Y. T._ Chem. Commun. 2015, 51, 9336-9338.

■ **ORDER**

**International**

**USD 350 + VAT (10%) + Fedex delivery charge**

**In Korea**

**KRW 450,000 + VAT (10%)**

**100 nmol** (dried form: equivalent to 1 mM / 100 µL)

[order@senprobe.com](mailto:order@senprobe.com)

■ **Storage**
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

■ **General Use Guide**

- More than 1/100 dilution of 10mM of DMSO stock solution is essential

-  For biomedical use to avoid DMSO concentration higher than 1%.

- Working concentrations for specific applications should be determined by the investigator.

- It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
- precipitated out from buffer solution.

■  **Data sheet PDF file**

CDb12 data sheet v0.2.pdf

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