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Nucleolus (RNA) probes

E36 [Catalog # P021]
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🔬PHOENIX FLUOR

E36 [Catalog # P021]

■ Chemical structure & Information
E36 is a selective RNA probe over DNA. E36 was disclosed through in vitro DNA/RNA selectivity screening and live cell staining for nucleolus
■ Cell Image
Live Cell RNA Staining with Selected Dyes E36, E144, and F22 were tested at a 5 mM concentration. The picture of F22-stained 3T3 cells was obtained in a 1 mM dye concentration.
1000x magnification was utilized in the imaging. The scale bar represents 5 mm. Image brightness and contrast were slightly adjusted to improve picture quality.
E36, E144 (green: FITC channel), and F22 (red: Cy3 channel) are shown.
Related probes: E144, F22
Known Property
RNA probe
Application
Selective fluorescent imaging of nuclear structure in live cells
Cell selectivity mechanism
RNA
Reference
1.
RNA-selective, live cell imaging probes for studying nuclear structure and function., Li, Q., Kim, Y. K., Namm, J., Kulkarni, A., Rosania, G., Ahn, Y. H., Chang, Y. T.* Chem. Biol. 2006, 13, 615-623.
2.
A protocol for preparing, characterizing and using three RNA-specific, live cell imaging probes: E36, E144 and F22 Li, Q.; Chang, Y. T.* Nat. Protoc. 2006, 1, 2922-2932.
3.
Nuclear envelope budding enables large ribonucleoprotein particle export during synaptic
wnt signaling
, Speese, S. D.; Ashley, J.; Jokhi, V.; Nunnari, J.; Barria, R.; Li, Y.; Ataman, B.; Koon, A.; Chang, Y. T.; Li, Q.; Moore, M. J.; Budnik, V. Cell 2012, 149, 832-846.

ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equvalent to 1 mM / 100 µL)

Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

General Use Guide
More than 1/100 dilution of 10mM of DMSO stock solution is essential
For biomedical use to avoid DMSO concentration higher than 1%.
Working concentrations for specific applications should be determined by the investigator.
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
Data sheet PDF file
E36 data sheet v0.2.pdf649KB
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