E144 is a selective RNA probe over DNA. E144 was disclosed through in vitro DNA/RNA selectivity screening and live cell staining for nucleolus
■ Cell Image
Live Cell RNA Staining with Selected Dyes E36, E144, and F22 were tested at a 5 mM concentration. The picture of F22-stained 3T3 cells was obtained in a 1 mM dye concentration. 1000x magnification was utilized in the imaging. The scale bar represents 5 mm. Image brightness and contrast were slightly adjusted to improve picture quality. E36, E144 (green: FITC channel), and F22 (red: Cy3 channel) are shown.
■ Related probes: E36, F22
■ Known Property RNA probe ■ Application Selective fluorescent imaging of nuclear structure in live cells ■ Cell selectivity mechanism RNA
■ Reference
1.
RNA-selective, live cell imaging probes for studying nuclear structure and function., Li, Q., Kim, Y. K., Namm, J., Kulkarni, A., Rosania, G., Ahn, Y. H., Chang, Y. T.* Chem. Biol. 2006, 13, 615-623.
2.
A protocol for preparing, characterizing and using three RNA-specific, live cell imaging probes: E36, E144 and F22 Li, Q.; Chang, Y. T.* Nat. Protoc. 2006, 1, 2922-2932.