Check out our newly launched website!
Go to homepage
Neutrophil probe
NeutropG [Catalog # P055]
■ Chemical structure & Information
NeutropG is a selective fluorescent probe for identifying and imaging live neutrophils, showing high specificity among granulocytes. It targets lipid droplet biosynthesis enzymes (ACSL and DGAT) following the Metabolism-Oriented Live-cell Distinction (MOLD) concept. NeutropG is fast, stable, wash-free, and biocompatible, proving effective in clinical blood samples and demonstrating potential as a diagnostic tool for metabolically active neutrophils
■ Cell Image
Fluorescence images showed the intracellular fluorescent intensity of neutrophils, eosinophils and basophils after treatment with NeutropG (0.2 μM), Nile Red (1 nM) and Hoechst (1 μg mL−1) for 1 h, 30 min and 10 min, respectively. The specific antibody for each cell type was added to cells and incubated for 30 min prior to imaging.
■ Known Property
Neutrophil probe
■ Application
Immunofluorescence
■ Cell selectivity mechanism
MOLD
Neutrophil probe
■ Application
Immunofluorescence
■ Cell selectivity mechanism
MOLD
■ Reference
1.
Neutrophil Selective Fluorescent Probe Development through Metabolism-Oriented Live-cell Distinction, Gao, M.; Lee, S. H.; Park, S. H.; Ciaramicoli, L. M.; Kwon, H. Y.; Cho, H.; Jeong, J.; Chang, Y. T.* Angew. Chem. Int. Ed. Engl. 2021, 60, 23742-23749.
■ ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equivalent to 1 mM / 100 µL)
■ Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C
■ General Use Guide
•
More than 1/100 dilution of 10mM of DMSO stock solution is essential
•
For biomedical use to avoid DMSO concentration higher than 1%.
•
Working concentrations for specific applications should be determined by the investigator.
•
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
precipitated out from buffer solution.
■ Data sheet PDF file
NeutropG data sheet v0.2.pdf801.48KB