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Neutrophil probe

NeutropG [Catalog # P055]
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🔬PHOENIX FLUOR

NeutropG [Catalog # P055]

■ Chemical structure & Information
NeutropG is a selective fluorescent probe for identifying and imaging live neutrophils, showing high specificity among granulocytes. It targets lipid droplet biosynthesis enzymes (ACSL and DGAT) following the Metabolism-Oriented Live-cell Distinction (MOLD) concept. NeutropG is fast, stable, wash-free, and biocompatible, proving effective in clinical blood samples and demonstrating potential as a diagnostic tool for metabolically active neutrophils
■ Cell Image
Fluorescence images showed the intracellular fluorescent intensity of neutrophils, eosinophils and basophils after treatment with NeutropG (0.2 μM), Nile Red (1 nM) and Hoechst (1 μg mL−1) for 1 h, 30 min and 10 min, respectively. The specific antibody for each cell type was added to cells and incubated for 30 min prior to imaging.
Known Property
Neutrophil probe
Application
Immunofluorescence
Cell selectivity mechanism
MOLD
Reference
1.
Neutrophil Selective Fluorescent Probe Development through Metabolism-Oriented Live-cell Distinction, Gao, M.; Lee, S. H.; Park, S. H.; Ciaramicoli, L. M.; Kwon, H. Y.; Cho, H.; Jeong, J.; Chang, Y. T.* Angew. Chem. Int. Ed. Engl. 2021, 60, 23742-23749.

ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equvalent to 1 mM / 100 µL)

Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

General Use Guide
More than 1/100 dilution of 10mM of DMSO stock solution is essential
For biomedical use to avoid DMSO concentration higher than 1%.
Working concentrations for specific applications should be determined by the investigator.
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
Data sheet PDF file
NeutropG data sheet v0.2.pdf821KB
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