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Neuronal stem cell probes
CDg13 [Catalog # P013]
■ Chemical structure & Information
CDg13 (Compound of Designation green 13) is a selective mouse neural stem cell (NSC) probe over mouse embryonic stem cell (mES), mouse embryonic fibroblast (MEF), and astrocyte. The mechanism of CDg13 for NSC selectivity was elucidated as gating-oriented live-cell distinction (GOLD) through ABCG2.
■ Cell Image
(a) Live cells were stained with 1mm CDg13 and Hoechst33342 for 1h. After brief washing in serum-free medium, fluorescence images of MEF, mESC, NS5,and differentiated NS5 (D-NS5) were taken. Scale bar: 50mm. (b) Histogram of CDg13-treated cells after flow cytometry.
■ Related probes: CDy5, CDr3
■ Known Property
mouse neural stem cell (NSC) probe
■ Application
Immunofluorescence
■ Cell selectivity mechanism
GOLD (ABCG2)
mouse neural stem cell (NSC) probe
■ Application
Immunofluorescence
■ Cell selectivity mechanism
GOLD (ABCG2)
■ Reference
1.
A Fluorescent Probe for Neural Stem/Progenitor Cells with High Differentiation Capability into Neurons, Kim, B.; Feng, S.; Yun, S. W.; Leong, C.; Satapathy, R.; Wan, S. Y.; Chang, Y. T.* ChemBioChem 2016, 17, 2118-2122.
■ ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equivalent to 1 mM / 100 µL)
■ Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C
■ General Use Guide
•
More than 1/100 dilution of 10mM of DMSO stock solution is essential
•
For biomedical use to avoid DMSO concentration higher than 1%.
•
Working concentrations for specific applications should be determined by the investigator.
•
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
precipitated out from buffer solution.
■ Data sheet PDF file
CDg13 data sheet v0.2.pdf640.44KB