Home
SENPRO
ABOUT US
🔬PROBES
FAQ
Subscribe

Neuronal stem cell probes

CDr3 [STEMCELL Technologies]
Copyright© All Rights Reserved.
🔬PHOENIX FLUOR

CDy5 [Catalog # P005]

Chemical structure & Information
CDy5 selectively stains neural stem cells in neurosphere. In symmetric division, two identical daughter cells were visualized by CDy5. In asymmetric division, only small stem cell is fluorescently labeled and the other bigger differentiated cell was not stained by CDy5. The binding target of CDy5 is acid ceramidase
Cell Image
(a) Confocal fluorescence micrograph (taken using A1R+ si, Nikon) of a CDy5-stained neuro sphere fixed in paraformaldehyde. Scale bar, 10 mm. (b) Chemical structure of CDy5. (c) Epifluorescence micrograph of living single cells dissociated fromCDy5-stainedneurospheres.CDy5-stainedcells are marked by white arrows. Scale bar, 10mm.(d) Neuro sphere assay of FACS sortedCDy5bright and CDy5 dim cells. Data represent mean SD. * p < 0.01, Student’s t-test.
Related probes: CDy1, CDr3
Known Property
neural stem cells dye
Application
Immunofluorescence
Cell selectivity mechanism
POLD (acid ceramidase)
Reference
1.
A fluorescent probe for imaging symmetric and asymmetric cell division in neurosphere formation, Yun, S. W.; Leong, C.; Bi, X.; Ha, H. H.; Yu, Y. H.; Tan, Y. L.; Narayanan, G.; Sankaran, S.; Kim, J. Y.; Hariharan, S.; Ahmed, S.; Chang, Y. T. Chem. Commun. 2014, 50, 7492-7494.

ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equvalent to 1 mM / 100 µL)

Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

General Use Guide
More than 1/100 dilution of 10mM of DMSO stock solution is essential
For biomedical use to avoid DMSO concentration higher than 1%.
Working concentrations for specific applications should be determined by the investigator.
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
Data sheet PDF file
CDy5 data sheet v0.2.pdf632KB
Made with SlashPage