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Microglia cell probes
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🔬PHOENIX FLUOR
CDr10a [Catalog # P010a]
■ Chemical structure & Information
CDr10 (Compound of Designation red 10) is microglia selective probe, and stains both primary microglia and mouse cell line BV2 cells. CDr10a is with acetyl group and CDr10b is with chloroacetyl group, which can make covalent bond with thiol group. Therefore, CDr10b staining survives cell methanol fixing condition, while CDr10a signal is washed out. CDr10 stains cytosol of microglia cell, and the selectivity mechanism is not known. CDr10b stains activated microglia stronger than resting state microglia. The low toxicity of CDr10b allowed a movie of microglia attacking glioma cells
■ Cell Image
CDr10b cell staining was relatively resistant to washing with methanol and media whereas CDr10a staining was completely washed out.
■ Related probes: CDr20
■ Known Property
Primary microglia and mouse cell line, BV2 cell selective probe
■ Application
Immunofluorescence
■ Cell selectivity mechanism
unknown
Primary microglia and mouse cell line, BV2 cell selective probe
■ Application
Immunofluorescence
■ Cell selectivity mechanism
unknown
■ Reference
1.
Microglia specific fluorescent probe for live cell imaging, Leong, C.; Lee, S. C.; Ock, J.; See, P.; Park, S. J.; Ginhoux, F.; Yun, S. W.; Chang, Y. T. Chem. Commun. 2014, 50, 1089-1091.
■ ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equvalent to 1 mM / 100 µL)
■ Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C
■ General Use Guide
•
More than 1/100 dilution of 10mM of DMSO stock solution is essential
•
For biomedical use to avoid DMSO concentration higher than 1%.
•
Working concentrations for specific applications should be determined by the investigator.
•
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
precipitated out from buffer solution.
■ Data sheet PDF file
CDr10a data sheet v0.2.pdf386KB