CDgB [Catalog # P032]

Fri, 17 Jan 2025 14:32:23 GMT

**■ Chemical structure & Information**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/222820_URYyb5kz8BXwlU7gFi?q=80&s=1280x180&t=outside&f=webp)

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/222834_kGWiIEfrbXrI2GRgIx?q=80&s=1280x180&t=outside&f=webp)

**CDgB** (Compound of Designation green for B cell) is a selective fluorescent probe for B cell over T cell. B and T lymphocytes are similar in size and shape and it is practically impossible to distinguish them without the aid of antibody. **CDgB** is the first small molecule probe which distinguish B cell from T cell. **CDgB** has long hydrocarbon chain and the cell selectivity originates from the cell membrane lipid composition. In comparing to B cell, T cell has longer hydrocarbon in the plasma membrane lipid and also higher contents of cholesterol.

**■ Cell Image**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/222903_kGYWf26ua41esg2Z84?q=80&s=1280x180&t=outside&f=webp)

Development of the B-cell-selective probe CDgB. (a) Fluorescence images of double staining for CDgB (left) and anti-CD3e (middle). The images (right) are merged with CDgB (FITC) and anti-CD3e (Cy5). The splenocytes that CDgB stains are not overlapped with anti-CD3e for T cells. Scale bar: 10 μm. (b) Flow cytometry dot-plot image of double staining with anti-CD3e (right) for T cells and CDgB (FITC) on splenocytes.

■ **Known Property**
B lymphocyte probe
■ **Application**
Immunofluorescence
■ **Cell selectivity mechanism**
LOLD (lipid membrane fluidity)

■ **Reference**

1. **Lipid-Oriented Live-Cell Distinction of B and T Lymphocytes**, Kwon, H. Y.; Kumar Das, R.; Jung, G. T.; Lee, H. G.; Lee, S. H.; Berry, S. N.; Tan, J. K. S.; Park, S.; Yang, J. S.; Park, S.; Baek, K.; Park, K. M.; Lee, J. W.; Choi, Y. K.; Kim, K. H.; Kim, S.; Kim, K. P.; Kang, N. Y._; Kim, K._; Chang, Y. T.* J. Am. Chem. Soc. 2021, 143, 5836-5844.

■ **ORDER**

**International**

**USD 350 + VAT (10%) + Fedex delivery charge**

**In Korea**

**KRW 450,000 + VAT (10%)**

**100 nmol** (dried form: equivalent to 1 mM / 100 µL)

[order@senprobe.com](mailto:order@senprobe.com)

■ **Storage**
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

■ **General Use Guide**

- More than 1/100 dilution of 10mM of DMSO stock solution is essential

- For biomedical use to avoid DMSO concentration higher than 1%.

- Working concentrations for specific applications should be determined by the investigator.

- It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
- precipitated out from buffer solution.

■ **Data sheet PDF file**

CDgB data sheet v0.2.pdf