CDb8 (Compound of Designation blue 8) is a xanthone based blue fluorescent probe for mouse embryonic stem cell (mES). CDb8 stains the outside of mES colony, i.e. glycocalyx area [1]. Glycocalyx is enriched with glycoproteins and glycolipids. The co-staining with related ES probes showed that CDy1 stains inside ES colony, and CDg4 and CDb8 stains the surface of ES colony [2]. Based on the imaging data, the binding target of CDb8 may be glycogen in glycocalyx.
■ Cell Image
Selective staining of mESC by CDb8. (a) Chemical structure of CDb8; (b) mESC was selectively stained by CDb8 at 1 mM for 1 h. Upper panel: mouse embryonic fibroblasts (MEF), middle: mouse embryonic stem cells (mESC), lower panel: mESC on MEF feeder. (c) Flow cytometry analysis of DMSO control cells. (d) Flow cytometry analysis of CDb8 stained cells. The cells are loaded after 1 h incubation at 1 mM. B.F: bright field, scale bar: 100 mm.
Solid phase combinatorial synthesis of xanthone library using click chemistry and its application to embryonic stem cell probe, Ghosh, K. K; Ha, H. H.; Kang, N. Y.; Chandran, Y.; Chang, Y. T.* Chem. Commun. 2011, 47, 7488-7490
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Development of fluorescent Chalcone library and its application in the discovery of a mouse embryonic stem cell probe, Lee, S. C.; Kang, N. Y.; Park, S. J.; Yun, S. W.; Chandran, Y.; Chang, Y. T.* Chem. Commun. 2012, 48, 6681-6683.