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Embryonic stem cell probes

CDy1 [Active Motif]
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🔬PHOENIX FLUOR

CDy9 [Catalog # P009]

Chemical structure & Information
CDy9 (Compound of Designation yellow 9) is a highly selective mouse embryonic stem cell (mES) probe. The selectivity of CDy9 was reduced when the chloroacetyl group was removed, while acetyl derivative maintained the selectivity.
Cell Image
CDy9 is a novel and versatile mESC-specific fluorescent probe. (a)Chemical structure of CDy9. (b) CDy9 selectively stains mESC over MEF. Both mESC and MEF were incubated with 1 mM CDy9 and imaged under the fluorescence microscope after 1 h. Scale bar: 25mm. (c) Flow cytometry analysis of mESC and MEF after incubation with CDy9. The fluorescence intensity of mESC upon treatment with CDy9 is brighter than in MEF (right dot plot) and in unstained mESC (left dot plot).
Related probes: CDy1, CDr3, CDb8
Known Property
mouse embryonic stem cell probe
Application
Immunofluorescence
Cell selectivity mechanism
GOLD (SLC13A5)
Reference
1.
A highly selective fluorescent probe for direct detection and isolation of mouse embryonic stem cells, Chandran, Y.; Kang, N. Y.; Park, S. J.; Husen, A. S.; Kim, J. Y.; Sahu, S.; Su, D.; Lee, J.; Vendrell, M.; Chang, Y. T. Bioorg. Med. Chem. Lett. 2015, 25, 4862-4865.
2.
A fluorescent chemical probe CDy9 selectively stains and enables the isolation of live naïve mouse embryonic stem cells, Cho, S. J.; Kim, K. T.; Kim, J. S.; Kwon, O. S.; Go, Y. H.; Kang, N. Y.; Heo, H.; Kim, M. R.; Han, D. W.; Moon, S. H.; Chang, Y. T.; Cha, H. J. Biomaterials 2018, 180, 12-23.

ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equvalent to 1 mM / 100 µL)

Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

General Use Guide
More than 1/100 dilution of 10mM of DMSO stock solution is essential
For biomedical use to avoid DMSO concentration higher than 1%.
Working concentrations for specific applications should be determined by the investigator.
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
Data sheet PDF file
CDy9 data sheet v0.2.pptx.pdf169KB
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