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Embryonic stem cell probes

CDy1 [Active Motif]
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🔬PHOENIX FLUOR

CDg4 [Catalog # P004]

Chemical structure & Information
CDg4 (Compound of Designation green 4) is a chalcone based green fluorescent probe for mouse embryonic stem cell (mES). CDg4 stains the outside of mES colony, i.e. glycocalyx area. Glycocalyx is enriched with glycoproteins and glycolipids. The binding target of Cdg4 was identified as glycogen in glycocalyx, and the CDg4 staining was diminished by amylase treatnebt. In contrast to mES colony, neurosphere lacks glycogen and is not stained by CDg4. The co-staining with related ES probes showed that CDy1 stains inside ES colony, and CDg4 and CDb8 stains the surface of ES colony
Cell Image
(a) The mESCs were co-cultured on MEF. The bright field (left) and fluorescent image (right) of CDg4. Scale bar: 200 mm. Images of stained cell colonies were taken by 4 objective lenses. (b) Flow cytometry dot plot images of mESC and MEF stained with DMSO as a control and CDg4 (lab: 430 nm, lem: 560 nm, e: 23 600, QY: 0.2 in DMSO).
Related probes: CDy1, CDb8, CDy9
Known Property
mouse embryonic stem cell (mES) probe
Application
Immunofluorescence
Cell selectivity mechanism
COLD (glycogen)
Reference
1.
Development of fluorescent Chalcone library and its application in the discovery of a mouse embryonic stem cell probe, Lee, S. C.; Kang, N. Y.; Park, S. J.; Yun, S. W.; Chandran, Y.; Chang, Y. T.* Chem. Commun. 2012, 48, 6681-6683.

ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equvalent to 1 mM / 100 µL)

Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

General Use Guide
More than 1/100 dilution of 10mM of DMSO stock solution is essential
For biomedical use to avoid DMSO concentration higher than 1%.
Working concentrations for specific applications should be determined by the investigator.
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
Data sheet PDF file
CDg4 data sheet v0.2.pdf630KB
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