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Nucleus (DNA) probes

DNA-green [Catalog # P033]
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🔬PHOENIX FLUOR

CDb12 [Catalog # P012]

■ Chemical structure & Information
CDb12 (Compound of Designation blue 12) was discovered from mitotic cell selective dye screening in RPE1 cells, and stains mitotic cells 7 times stronger than interphase cells. CDyb12 stains nucleus and increase the fluorescence upon treatment of DNA, but not of RNA. CDb12 could monitor the dynamic cell division at least for 2 cell division cycle. In contrast, Hoechst treated cells showed cell cycle arrest at M phase, may be due to too strong binding to DNA
■ Cell Image
Time-lapse imaging of CDb12, compared to Hoechst 33342. RPE1 cells were stained with CDb12 (1 mM) or Hoechst 33342 (0.5 mg mL1 ). The mitosis was monitored for 24 h with frames taken every 3 min on a timelapse imaging system Nikon Biostation IM (Nikon) with DAPI filter (405 nm).
Related probes: CDy6
Known Property
mitotic cell probe
Application
Immunofluorescence
Cell selectivity mechanism
Unknown, neuclear localization
Reference
1.
Development of nucleus staining fluorescent probe for dynamic mitosis imaging in live cells, Ghosh, K. K.; Jeong, Y. M.; Kang, N. Y.; Lee, J. Y.; Wan, S. Y.; Kim, J. Y.; Yoo, J.; Kim, D.; Kim, Y. K.; Chang, Y. T. Chem. Commun. 2015, 51, 9336-9338.

ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equvalent to 1 mM / 100 µL)

Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

General Use Guide
More than 1/100 dilution of 10mM of DMSO stock solution is essential
For biomedical use to avoid DMSO concentration higher than 1%.
Working concentrations for specific applications should be determined by the investigator.
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
Data sheet PDF file
CDb12 data sheet v0.2.pdf633KB
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