CO-1 [Catalog # P049]

Mon, 20 Jan 2025 12:25:50 GMT

**■ Chemical structure & Information**

![Image](https://upload.cafenono.com/image/slashpagePost/20250919/101859_wCvumfPMv2cmnf6t5L?q=80&s=1280x180&t=outside&f=webp)

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/220238_bA3pIBv8h8kPn51vbd?q=80&s=1280x180&t=outside&f=webp)

**CO-1** is cell permeable background-free probe through optimized lipophilicity, water solubility and charged van der Waals surface area. **CO-1**, a cyclooctyne-containing BODIPY probe, is also specifically label intracellular azide-tagged organelles & engineered proteins with minimum background.

**■ Cell Image**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/220313_FACsPDQuhJ4NXXPaMH?q=80&s=1280x180&t=outside&f=webp)

Fluorescence imaging of mitochondria, lysosome and golgi apparatus in U-2 OS cells labelled with **CO-1**. Cells pre-treated with TPP-Az, Morph-Az or Sphingo-Az were incubated with 2 μM CO-1 in growth media at 37 °C for 1 h and followed by counterstaining with organelle trackers. Scale bar, 15 μm. (iv) Fluorescence imaging of histone H2B in live U-2 OS. U-2 OS cells were co-transfected with plasmids pIre-Azi3 and pmH2B-6-mKate2-16tag to incorporate UAA Azi into H2B-mKate2 at site 16. After Azi incorporation, cells were labelled with 10 μM CO-1 for 90 min at 37 °C. Cells were then washed and imaged. The mKate2 (red) signals were detected in cell nuclei only, and colocalized with the **CO-1** (green) signals in the merged image. Scale bar, 5 μm.

■ **Related probes:** AzG-1

■ **Known Property**
Bio-orthogonal Click reaction
■ **Application**
Immunofluorescence
■ **Cell selectivity mechanism**
unknown

■ **Reference**

1. **Development of background-free tame fluorescent probes for intracellular live cell imaging**, Alamudi, S. H.; Satapathy, R.; Kim, J.; Su, D.; Ren, H.; Das, R.; Hu, L.; Alvarado-Martínez, E.; Lee, J. Y.; Hoppmann, C.; Peña-Cabrera, E.; Ha, H. H.; Park, H. S._; Wang, L._; Chang, Y. T.* Nat. Commun. 2016, 7, 11964.

■ **ORDER**

**International**

**USD 350 + VAT (10%) + Fedex delivery charge**

**In Korea**

**KRW 450,000 + VAT (10%)**

**100 nmol** (dried form: equivalent to 1 mM / 100 µL)

[order@senprobe.com](mailto:order@senprobe.com)

■ **Storage**
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

■ **General Use Guide**

- More than 1/100 dilution of 10mM of DMSO stock solution is essential

- For biomedical use to avoid DMSO concentration higher than 1%.

- Working concentrations for specific applications should be determined by the investigator.

- It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
- precipitated out from buffer solution.

■ **Data sheet PDF file**

CO-1 data sheet v0.2.pdf