# CDy2 [Catalog # P002]

**■ Chemical structure & Information**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/222110_XbSYKfbs0Wf2c8olhL?q=80&s=1280x180&t=outside&f=webp)

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/222127_rPT5OK6hcJkFawlG1e?q=80&s=1280x180&t=outside&f=webp)

**CDy2** stains mitochondria in live muscle cells and target protein was expected to covalently labeled through CA group of **CDy2**. The fluorescent property of **CDy2 **allowed to monitor the binding protein from SDS-gel and the biomarker of **CDy2** was elucidated as ALDH2.

**■ Cell Image**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/222157_KX4aqHutmKOc3dYriR?q=80&s=1280x180&t=outside&f=webp)

Identification of protein binders in vitro and in living cells. (a) Chemical structure of** CDy2** for labeling a target protein in living cells. (b) Myoblasts (MB) or myotubes (MT) were incubated with **CDy2 **(500 nM) for 30 min and imaged with a fluorescent microscope. Then, cells were lysed for in-gel fluorescence analysis (lex=530 nm, lex=580 nm) (c) Western blot analysis (WB). (d) Two-dimensional gel analysis for the identification of the labeled protein.

■ **Related probes**: E26, I25, I31

■ **Known Property**
differentiated muscle cell probe
■ **Application**
Immunofluorescence
■ **Cell selectivity mechanism**
POLD (ALDH2 in mitochondria)

■ **Reference**

1. **Binding of fluorophores to proteins depends on the cellular environment**, Kim, Y. K.; Lee, J. S.; Bi, X.; Ha, H. H.; Ng, S. H.; Ahn, Y. H.; Lee, J. J.; Wagner, B. K.; Clemons, P. A.; Chang, Y. T.* Angew. Chem., Int. Ed. Engl. 2011, 50, 2761-2763.

2. **Small-molecule fluorophores to detect cell-state switching in the context of highthroughput screening**, Wagner, B. K.; Carrinski, H. A.; Ahn, Y. H.; Kim, Y. K.; Gilbert, T. J.; Fomina, D. A.; Schreiber, S. L.; Chang, Y. T.; Clemons, P. A. J. Am. Chem. Soc. 2008, 130, 4208-4209.

3. **Site-selective labeling at Cys302 of aldehyde dehydrogenase unveils a selective mitochondrial stain**, Kim, Y. K.; Lee, J. K.; Lee, J. S.; Yoon, C. N.; Chang, Y. T.* Mol. Biosyst. 2011, 7, 2375-2378.

■ **ORDER**

**International**

**USD 350 + VAT (10%) + Fedex delivery charge**

**In Korea**

**KRW 450,000 + VAT (10%)**

**100 nmol** (dried form: equivalent to 1 mM / 100 µL)

[order@senprobe.com](mailto:order@senprobe.com)

■ **Storage**
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

■ **General Use Guide**

- More than 1/100 dilution of 10mM of DMSO stock solution is essential

-  For biomedical use to avoid DMSO concentration higher than 1%.

- Working concentrations for specific applications should be determined by the investigator.

- It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
- precipitated out from buffer solution.

■** Data sheet PDF file**

CDy2 data sheet v0.2.pptx.pdf

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