# CDg4 [Catalog # P004]

■ **Chemical structure & Information**

![Image](https://upload.cafenono.com/image/slashpagePost/20250805/190720_cBs5hoeqT4urrrVpIN?q=80&s=1280x180&t=outside&f=webp)

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/211922_mVrDQVjPEQMjDwZS9H?q=80&s=1280x180&t=outside&f=webp)

**CDg4** (Compound of Designation green 4) is a chalcone based green fluorescent probe for mouse embryonic stem cell (mES). **CDg4** stains the outside of mES colony, i.e. glycocalyx area. Glycocalyx is enriched with glycoproteins and glycolipids. The binding target of Cdg4 was identified as glycogen in glycocalyx, and the **CDg4** staining was diminished by amylase treatnebt. In contrast to mES colony, neurosphere lacks glycogen and is not stained by **CDg4**. The co-staining with related ES probes showed that CDy1 stains inside ES colony, and **CDg4** and CDb8 stains the surface of ES colony

■ **Cell Image**

![Image](https://upload.cafenono.com/image/slashpagePost/20250206/212236_fFtPoSDdcNqnUAFqdF?q=80&s=1280x180&t=outside&f=webp)

(a) The mESCs were co-cultured on MEF. The bright field (left) and fluorescent image (right) of CDg4. Scale bar: 200 mm. Images of stained cell colonies were taken by 4 objective lenses. (b) Flow cytometry dot plot images of mESC and MEF stained with DMSO as a control and CDg4 (lab: 430 nm, lem: 560 nm, e: 23 600, QY: 0.2 in DMSO).

■ **Related probes**: CDy1, CDb8, CDy9

■ **Known Property**
mouse embryonic stem cell (mES) probe

■ **Application**
Immunofluorescence

■ **Cell selectivity mechanism**
COLD (glycogen) 

■ **ORDER**

**International**

**USD 350 + VAT (10%) + Fedex delivery charge**

**In Korea**

**KRW 450,000 + VAT (10%)**

**100 nmol** (dried form: equivalent to 1 mM / 100 µL)

[order@senprobe.com](mailto:order@senprobe.com)

■ **Storage**
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

■ **General Use Guide**

- More than 1/100 dilution of 10mM of DMSO stock solution is essential

-  For biomedical use to avoid DMSO concentration higher than 1%.

- Working concentrations for specific applications should be determined by the investigator.

- It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
- precipitated out from buffer solution.

■ **Data sheet PDF file**

CDg4 data sheet.pdf

■ **Reference**

1. **Development of fluorescent Chalcone library and its application in the discovery of a mouse embryonic stem cell probe**, Lee, S. C.; Kang, N. Y.; Park, S. J.; Yun, S. W.; Chandran, Y.; Chang, Y. T.* Chem. Commun. 2012, 48, 6681-6683.

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