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Bioflim probes

CDy11 - Amyloid targeting [Patent pending]
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🔬PHOENIX FLUOR

CDr15 - DNA tageting [Patent pending]

■ Chemical structure & Information
Bacterial biofilm is a self-generated extracellular polymeric substance (EPS), composed of extracellular DNA, polysaccharides, and proteins such as amyloids. CDr15 (Compound of Designation red 15) is discovered through an unbiased bacterial biofilm screening with cyclic di-GMP as the biofilm turn-on switch.
■ Cell Image
Localization of CDr15 on biofilm with different development times. (A) Different localization of CDr15 on biofilm showed at different forming times. PAO1-GFP; wild type P. aeruginosa was cultured in a chamber slide for 1, 3, and 5 days. CDr15 was applied to biofilm with different development times. The images were taken using a confocal microscope. Scale bars, 10 μm. (B) The CDr15 stain patterns during the biofilm formation were shown in the schematic figure and vertical sectioned images and (black box). Scale bars, 10 μm.
Related probes: CDy11, CDr14
Known Property
extracellular DNA (eDNA) probe
Application
Immunofluorescence
Cell selectivity mechanism
DOLD (eDNA)
Known Property
extracellular DNA (eDNA) probe
Application
Immunofluorescence
Cell selectivity mechanism
DOLD (eDNA)
Reference
1.
Visualizing biofilm by targeting eDNA with long wavelength probe CDr15, Kwon, H. Y.; Kim, J. Y.; Liu, X.; Lee, J. Y.; Yam, J. K. H.; Dahl Hultqvist, L.; Xu, W.; Rybtke, M.; Tolker-Nielsen, T.; Heo, W.; Kim, J. J.; Kang, N. Y.; Joo, T.; Yang, L.; Park, S. J.; Givskov, M.; Chang, Y. T.* Biomater.
Sci. 2019, 7, 3594-3598.
2.
Validation of CDr15 as a new dye for detecting neutrophil extracellular trap, Kim, S. J.; Kim, J.; Kim, B.; Lee, W. W.; Liu, X.; Chang, Y. T.; Park, J. W. Biochem. Biophys. Res. Commun. 2020, 527, 646-653

ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equvalent to 1 mM / 100 µL)

Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

General Use Guide
More than 1/100 dilution of 10mM of DMSO stock solution is essential
For biomedical use to avoid DMSO concentration higher than 1%.
Working concentrations for specific applications should be determined by the investigator.
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
Data sheet PDF file
CDr15-data-sheet-v0.1-2.pdf616KB
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