CDr15 - DNA tageting [Patent pending]

Fri, 17 Jan 2025 14:34:59 GMT

![Image](https://upload.cafenono.com/image/slashpagePost/20250919/101748_tCTwXMkPe53OTTlgtO?q=80&s=1280x180&t=outside&f=webp)

![Image](https://upload.cafenono.com/image/slashpagePost/20250207/203811_lPEIqDc9UjX5JLUlIK?q=80&s=1280x180&t=outside&f=webp)

Bacterial biofilm is a self-generated extracellular polymeric substance (EPS), composed of extracellular DNA, polysaccharides, and proteins such as amyloids. CDr15 (Compound of Designation red 15) is discovered through an unbiased bacterial biofilm screening with cyclic di-GMP as the biofilm turn-on switch.

**■ Cell Image**

![Image](https://upload.cafenono.com/image/slashpagePost/20250207/203904_HDibbOa4pAtbIGqCYh?q=80&s=1280x180&t=outside&f=webp)

Localization of **CDr15** on biofilm with different development times. (A) Different localization of CDr15 on biofilm showed at different forming times. PAO1-GFP; wild type P. aeruginosa was cultured in a chamber slide for 1, 3, and 5 days. CDr15 was applied to biofilm with different development times. The images were taken using a confocal microscope. Scale bars, 10 μm. (B) The CDr15 stain patterns during the biofilm formation were shown in the schematic figure and vertical sectioned images and (black box). Scale bars, 10 μm.

■ **Related probes**: CDy11, CDr14
**Known Property**
extracellular DNA (eDNA) probe
■ **Application**
Immunofluorescence
■ **Cell selectivity mechanism**
DOLD (eDNA)

■ **Known Property**
extracellular DNA (eDNA) probe
■ **Application**
Immunofluorescence
■ **Cell selectivity mechanism**
DOLD (eDNA)

■ **Reference**

1. **Visualizing biofilm by targeting eDNA with long wavelength probe CDr15**, Kwon, H. Y.; Kim, J. Y.; Liu, X.; Lee, J. Y.; Yam, J. K. H.; Dahl Hultqvist, L.; Xu, W.; Rybtke, M.; Tolker-Nielsen, T.; Heo, W.; Kim, J. J.; Kang, N. Y.; Joo, T.; Yang, L.; Park, S. J._; Givskov, M._; Chang, Y. T.* Biomater.
1. Sci. 2019, 7, 3594-3598.

2. **Validation of CDr15 as a new dye for detecting neutrophil extracellular trap**, Kim, S. J.; Kim, J.; Kim, B.; Lee, W. W.; Liu, X.; Chang, Y. T.; Park, J. W. Biochem. Biophys. Res. Commun. 2020, 527, 646-653