CDy14 - Polysaccharide targeting [Patent pending]

Fri, 17 Jan 2025 14:35:30 GMT

![Image](https://upload.cafenono.com/image/slashpagePost/20250919/101654_fKGjUQSlfDayCnOM2y?q=80&s=1280x180&t=outside&f=webp)

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Bacterial biofilm is a self-generated extracellular polymeric substance (EPS), composed of extracellular DNA, polysaccharides, and proteins such as amyloids. CDy14 (Compound of Designation yellow 14) is discovered through an unbiased bacterial biofilm screening with cyclic-di-GMP as the biofilm turn-on switch.

**■ Cell Image**

![Image](https://upload.cafenono.com/image/slashpagePost/20250207/203530_DwjrDc97ovVbLo8e1P?q=80&s=1280x180&t=outside&f=webp)

Localization of **CDy14** on the biofilm of PAO1-GFP, wild type P. aeruginosa. (A) **CDy14** stained extracellular matrix of biofilm with images taken using CLSM or SR-SIM, respectively. Scale bar, 10 mm. (B) The different localizations of **CDy14** on the biofilm are shown at different forming times.
PAO1- GFP, wild type P. aeruginosa, was cultured in the chamber slide for 1, 3, and 5 days. **CDy14** was applied to the biofilm with different development times. The images were taken using the Nikon confocal microscope. Scale bar, 10 mm. The **CDy14 **stain patterns during the biofilm formation are shown in the vertical sectioned images and the schematic figure (blue box).

■ **Related probes**: CDy11, CDr15

■ **Known Property**
exopolysaccharide psl probe
■ **Application**
Immunofluorescence
■ **Cell selectivity mechanism**
COLD (psl exopolysaccharide)

■ **Reference**

1. **CDy14: a novel biofilm probe targeting exopolysaccharide Psl**, Kwon, H. Y.; Kim, J. Y.; Lee, J.
1. Y.; Yam, J. K. H.; Hultqvist, L. D.; Xu, W.; Rybtke, M.; Tolker-Nielsen, T.; Kim, J. J.; Kang, N. Y.; Yang, L.; Park, S. J._; Givskov, M._; Chang, Y. T.* Chem. Commun. 2018, 54, 11865-11868.