CDy11 - Amyloid targeting [Patent pending]

Fri, 17 Jan 2025 14:36:12 GMT

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Bacterial biofilm is a self-generated extracellular polymeric substance (EPS), composed of extracellular DNA, polysaccharides, and proteins such as amyloids. CDy11 (Compound of Designation yellow 11) is discovered through a screening for bacteria with functional amyloids in Pseudomonas aeruginosa (Fap).

**■ Cell Image**

![Image](https://upload.cafenono.com/image/slashpagePost/20250207/203147_RKDrh3Lg3S6F59LPJH?q=80&s=1280x180&t=outside&f=webp)

Evaluation of target of **CDy11.** (A) Transmission electron microscopic image of purified amyloid. Scale bar, 400 nm. (B) Surface plasma resonance assay with **CDy11 **and purified amyloid after immobilization on chips. Values were calculated by measurements for their response unit by flowing ThT and **CDy11**. (C) Co-localization of **CDy11** and Fap antibody. Biofilms were incubated with primary Fap antibody and secondary antibody linked with Cy5. Subsequently,** CDy11** was treated for 1 h before acquiring images. The images were captured using a fluorescence microscope equipped with ×100 objective lens. Scale bars, 10 μm. The images are shown in pseudocolors.

■ **Related probes**: CDy14, CDr15

■ **Known Property**
Bacterial amyloids probe
■ **Application**
Immunofluorescence
■ **Cell selectivity mechanism**
POLD (Bacterial amyloids)

■ **Reference**

1. **Detection of pathogenic biofilms with bacterial amyloid targeting fluorescent probe, CDy11**, Kim, J. Y.; Sahu, S.; Yau, Y. H.; Wang, X.; Shochat, S. G.; Nielsen, P. H.; Dueholm, M. S.; Otzen, D. E.; Lee, J.; Delos Santos, M. M.; Yam, J. K.; Kang, N. Y.; Park, S. J.; Kwon, H.; Seviour, T. W.; Yang, L.; Givskov, M.; Chang, Y. T.* J. Am. Chem. Soc. 2016, 138, 402-407.

2. **A mouse ear skin model to study the dynamics of innate immune responses against Staphylococcus aureus biofilms**, Abdul Hamid, A. I.; Nakusi, L.; Givskov, M.; Chang, Y. T.; Marquès, C.; Gueirard, P. BMC Microbiol. 2020, 20:22.