# AGN (Negative control of AGO) [Catalog # P057]

**■ Chemical structure & Information**

![Image](https://upload.cafenono.com/image/slashpagePost/20250919/100857_7frptelMdKtBrIowDS?q=80&s=1280x180&t=outside&f=webp)

![Image](https://upload.cafenono.com/image/slashpagePost/20250313/103936_eA1vgvK5q4KkAynh0E?q=80&s=1280x180&t=outside&f=webp)

**AGN **(Advanced Glycation end-products Negative) is a negative control probe designed to validate the selectivity of AGO, engineered to prevent AGEs binding and produce minimal fluorescence. AGN features a meta-methoxybenzyl substituent, which prevents interactions with Schiff bases and Amadori products, making it an ideal negative control. It shares the same fluorescence properties as AGO (Excitation 490 nm / Emission 570 nm) but does not emit significant fluorescence in the presence of AGEs. By comparing AGO and AGN staining, AGN helps distinguish true AGEs-specific fluorescence from nonspecific background signals, ensuring reliable AGEs detection.

**■ Image**

![Image](https://upload.cafenono.com/image/slashpagePost/20250313/093345_B8KcO2buOXAO3hydBy?q=80&s=1280x180&t=outside&f=webp)

AGO selectivity to AGEs derived from the collagen model. The collagen model was utilized to confirm the selectivity of AGO. The fabricated collagen was incubated with 500 mM ribose for 1 week to form the glycation. The collagen hydrogel was stained using an anti-collagen I antibody, and then stained using (A)** AGN** (negative control) and (B) AGO.

■ **Known Property**
AGE-selective negative probe
■ **Application**
fluorescence image
■ **Cell selectivity mechanism**
unknown

■ **Reference**

1. **Development of a specific fluorescent probe to detect advanced glycation end products (AGEs)**, Cho, H.; Hong, N.-K.; Yong, I.; Kwon, H.-Y.; Kang, N.-Y.; Ciaramicoli, L. M.; Kim, P.; Chang, Y.-T.* J. Mater. Chem. B 2024, 12, 6155–6163.

■ **ORDER**

**International**

**USD 350 + VAT (10%) + Fedex delivery charge**

**In Korea**

**KRW 450,000 + VAT (10%)**

**100 nmol** (dried form: equivalent to 1 mM / 100 µL)

[order@senprobe.com](mailto:order@senprobe.com)

■ **Storage**
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

■ **General Use Guide**

- More than 1/100 dilution of 10mM of DMSO stock solution is essential

-  For biomedical use to avoid DMSO concentration higher than 1%.

- Working concentrations for specific applications should be determined by the investigator.

- It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
- precipitated out from buffer solution.

■ **Data sheet PDF file**

AGN data sheet.pdf

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