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Mitotic cell probes

CDy6 - Lysosome targeting [Catalog # P006]
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🔬PHOENIX FLUOR

CDy6 - Lysosome targeting [Catalog # P006]

■ Chemical structure & Information
CDy6 (Compound of Designation yellow 6) was discovered from mitotic cell selective dye screening. CDy6 stains lysosome and the fluorescence intensity increases during the mitosis in RPE1, Hela, and U2OS cells. CDy6 is photostable and non-toxic, so the stable lysosome imaging was possible for at least 3 cell division cycle
■ Cell Image
Lysosomal-Targeted CDy6 Labeling Effectively Visualizes Mitosis in Real-Time Live Imaging RPE1:H2B:GFP cells were stained with CDy6 (0.5 mM), and imaged for 8 hr. The H2B:GFP signal was used as a positive selection marker of cell division. Scalebars, 20 mm.
Related probes: CDb12
Known Property
mitotic cell probe
Application
Immunofluorescence
Cell selectivity mechanism
Unknown, lysosomal localization
Reference
1.
CDy6, a photostable probe for long-term real-time visualization of mitosis and proliferating cells, Jeong, Y. M.; Duanting, Z.; Hennig, H.; Samanta, A.; Agrawalla, B. K.; Bray, M. A.; Carpenter, A. E.; Chang, Y. T.* Chem. Biol. 2015, 22, 299-307.

ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equvalent to 1 mM / 100 µL)

Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C

General Use Guide
More than 1/100 dilution of 10mM of DMSO stock solution is essential
For biomedical use to avoid DMSO concentration higher than 1%.
Working concentrations for specific applications should be determined by the investigator.
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
Data sheet PDF file
CDy6 data sheet v0.2.pdf436KB
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