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CDb12 - Nucleus targeting [Catalog # P012]
■ Chemical structure & Information
CDb12 (Compound of Designation blue 12) was discovered from mitotic cell selective dye screening in RPE1 cells, and stains mitotic cells 7 times stronger than interphase cells. CDb12 stains nucleus and increase the fluorescence upon treatment of DNA, but not of RNA. CDb12 could monitor the dynamic cell division at least for 2 cell division cycle. In contrast, Hoechst treated cells showed cell cycle arrest at M phase, may be due to too strong binding to DNA
■ Cell Image
Time-lapse imaging of CDb12, compared to Hoechst 33342. RPE1 cells were stained with CDb12 (1 mM) or Hoechst 33342 (0.5 mg mL1 ). The mitosis was monitored for 24 h with frames taken every 3 min on a timelapse imaging system Nikon Biostation IM (Nikon) with DAPI filter (405 nm).
■ Related probes: CDy6
■ Known Property
mitotic cell probe
■ Application
Immunofluorescence
■ Cell selectivity mechanism
Unknown, neuclear localization
mitotic cell probe
■ Application
Immunofluorescence
■ Cell selectivity mechanism
Unknown, neuclear localization
■ Reference
1.
Development of nucleus staining fluorescent probe for dynamic mitosis imaging in live cells, Ghosh, K. K.; Jeong, Y. M.; Kang, N. Y.; Lee, J. Y.; Wan, S. Y.; Kim, J. Y.; Yoo, J.; Kim, D.; Kim, Y. K.; Chang, Y. T. Chem. Commun. 2015, 51, 9336-9338.
■ ORDER
International
USD 350 + VAT (10%) + Fedex delivery charge
In Korea
KRW 450,000 + VAT (10%)
100 nmol (dried form: equivalent to 1 mM / 100 µL)
■ Storage
① Delivery: Room Temperature
② Dried compound: 4°C or -20°C
③ Compound solution: 4°C or -20°C
■ General Use Guide
•
More than 1/100 dilution of 10mM of DMSO stock solution is essential
•
For biomedical use to avoid DMSO concentration higher than 1%.
•
Working concentrations for specific applications should be determined by the investigator.
•
It is recommended to use up the buffer diluted solution within one day. The compound may be decomposed or
precipitated out from buffer solution.
precipitated out from buffer solution.
■ Data sheet PDF file
CDb12 data sheet v0.2.pdf617.94KB