RESEARCH PROJECT

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circRNAs
Paper Reading
Pilot Study
Experimental methods
miRNA expression level
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
https://www.youtube.com/watch?v=guUSs-PyaCU
HB miR Multi Assay Kit (System I로 진행)
Western blot
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
protein expression level
Protein extraction
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
Protein quantification
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
Bradford assay
PCR cloning (for circRNA expression)
  1. circRNAs
  2. Experimental methods
  • J
    Jina Amie
BIOFACT
KPNA3 regulates histone locus body formation by modulating condensation and nuclear import of NPAT
  1. Paper Reading
  • J
    Jina Amie
https://rupress.org/jcb/article/224/1/e202401036/277130/KPNA3-regulates-histone-locus-body-formation-by?searchresult=1
circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis
  1. circRNAs
  2. Pilot Study
  3. Paper Reading
  • J
    Jina Amie
Not Started
Chen D, Zhang Y, Meng L, Lu L, Meng G. circRNA DENND1B inhibits tumorigenicity of clear cell renal cell carcinoma via miR-122-5p/TIMP2 axis. Open Med (Wars). 2022 Dec 16;17(1):2085-2097. doi: 10.1515/med-2022-0536. PMID: 36578555; PMCID: PMC9761921.
2월 저널클럽 발표 논문

KPNA3 regulates histone locus body formation by modulating condensation and nuclear import of NPAT

Category
  1. Paper Reading
Created by
  • J
    Jina Amie
Created at
URL
https://rupress.org/jcb/article/224/1/e202401036/277130/KPNA3-regulates-histone-locus-body-formation-by?searchresult=1
The histone locus body (HLB) is a membraneless organelle that determines the transcription of replication-dependent histones. However, the mechanisms underlying the appropriate formation of the HLB in the nucleus but not in the cytoplasm remain unknown. HLB formation is dependent on the scaffold protein NPAT. We identify KPNA3 as a specific importin that drives the nuclear import of NPAT by binding to the nuclear localization signal (NLS) sequence. NPAT undergoes phase separation, which is inhibited by KPNA3-mediated impairment of self-association. In this, a C-terminal self-interaction facilitator (C-SIF) motif, proximal to the NLS, binds the middle 431–1,030 sequence to mediate the self-association of NPAT. Mechanistically, the anchoring of KPNA3 to the NPAT-NLS sterically blocks C-SIF motif-dependent NPAT self-association. This leads to the suppression of aberrant NPAT condensation in the cytoplasm. Collectively, our study reveals a previously unappreciated role of KPNA3 in modulating HLB formation and delineates a steric hindrance mechanism that prevents inappropriate cytoplasmic NPAT condensation.
NPAT-KPNA3
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