HumAIne-chatbot is an AI-based conversational agent that provides personalized conversations based on user characteristics. It pre-trains various GPT-generated virtual personas to gain prior knowledge of a wide range of user types. During real-time interactions, it refines the user model by combining implicit signals (typing speed, emotions, engagement time) with explicit feedback (likes/dislikes) through a reinforcement learning agent. This profile is dynamically applied to the chatbot's conversational policies, adjusting content and style in real time. Controlled experiments with 50 synthetic personas showed that enabling personalization improved user satisfaction, personalization accuracy, and task accomplishment. Statistical analysis confirmed significant differences between personalized and non-personalized conditions.