This is a page that curates AI-related papers published worldwide. All content here is summarized using Google Gemini and operated on a non-profit basis. Copyright for each paper belongs to the authors and their institutions; please make sure to credit the source when sharing.
CRISPR-GPT for Agentic Automation of Gene-editing Experiments
Created by
Haebom
Author
Yuanhao Qu, Kaixuan Huang, Ming Yin, Kanghong Zhan, Dyllan Liu, Di Yin, Henry C. Cousins, William A. Johnson, Xiaotong Wang, Mihir Shah, Russ B. Altman, Denny Zhou, Mengdi Wang, Le Cong
Outline
CRISPR-GPT automates and enhances the design process for CRISPR-based genome editing experiments by adding domain knowledge and external tools to a large-scale language model (LLM). It supports the entire genome editing experiment process, including CRISPR system selection, guide RNA design, cell delivery method recommendation, protocol development, and validation experiment design to confirm editing results. It empowers even non-expert researchers to design experiments using CRISPR genome editing technology from scratch and validates its effectiveness through real-world use cases. It also discusses ethical and regulatory considerations related to automated genome editing design, emphasizing the need for responsible and transparent use of these tools.
Takeaways, Limitations
•
Takeaways:
◦
Improving accessibility of CRISPR-based gene editing experiments to non-expert researchers.
◦
Automating and increasing efficiency in the CRISPR experimental design process.
◦
Presenting the possibility of solving complex biological discovery problems based on LLM.
◦
Urging consideration of the ethical and regulatory aspects of gene editing technology.
•
Limitations:
◦
The lack of specific knowledge in LLM and the lack of clear solutions to the limitations of accuracy in solving biological design problems (further research is needed).
◦
Further validation of the practical applicability and generalizability of CRISPR-GPT is needed.
◦
A deeper discussion is needed on the ethical and regulatory issues surrounding automated gene editing systems.